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. 2021 Mar;53(2):e13900.
doi: 10.1111/and.13900. Epub 2020 Dec 1.

The effects of hesperidin on colistin-induced reproductive damage, autophagy, and apoptosis by reducing oxidative stress

Emrah Hicazi Aksu  1 , Fatih Mehmet Kandemir  2 , Sefa Küçükler  2
Affiliations

The effects of hesperidin on colistin-induced reproductive damage, autophagy, and apoptosis by reducing oxidative stress

Emrah Hicazi Aksu et al. Andrologia. 2021 Mar.

Abstract

This study has been conducted to investigate the effect of hesperidin on colistin-induced reproductive damage in male rats. Twenty-four adult male Sprague Dawley rats were used as animal material. They were divided into four groups: control group, received physiological saline for 7 days by oral gavage; hesperidin group, received 300 mg/kg day hesperidin for 7 days; colistin group, received 73 mg/kg (total dose) colistin during 7 days; and colistin + hesperidin group, received 300 mg/kg day hesperidin following the colistin treatment. At the end of the study, routine spermatological parameters and biochemical evaluations were assayed. Also, apoptosis and autophagy biomarkers in testes were evaluated. Colistin increased oxidative stress, apoptosis and autophagy expression levels in testis. Hesperidin supplementation significantly decreased the oxidative stress levels in the testes of the colistin + hesperidin group when compared to the colistin group. The highest apoptosis and autophagy expression levels were detected in the colistin group. These values were statistically lower in the colistin + hesperidin group when compared to the colistin group. Colistin treatment decreased the percentage of sperm motility and increased sperm abnormality. Hesperidin supplementation mitigated significantly mentioned side effects compared to the colistin group. In conclusion, hesperidin supplementation can be a good strategy to mitigate colistin-induced testicular toxicity.

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References

REFERENCES

    1. Aebi, H. (1983). Catalase. Methods in Enzymatic Analysis (pp. 276-286). Academic Press.
    1. Aksu, E. H., Akman, O., Ömür, A. D., Karakuş, E., Can, I., Kandemir, F. M., Dorman, E., & Uçar, Ö. (2016). 3, 3 diindolylmethane leads to apoptosis, decreases sperm quality, affects blood estradiol 17 β and testosterone, oestrogen (α and β), and androgen receptor levels in the reproductive system in male rats. Andrologia, 48(10), 1155-1165.
    1. Aksu, E. H., Kandemir, F. M., Altun, S., Küçükler, S., Çomaklı, S., & Ömür, A. D. (2016). Ameliorative effect of carvacrol on cisplatin-Induced reproductive damage in male rats. Journal of Biochemical and Molecular Toxicology, 30(10), 513-520.
    1. Aksu, E. H., Kandemir, F. M., Küçükler, S., & Mahamadu, A. (2018). Improvement in colistin-induced reproductive damage, apoptosis, and autophagy in testes via reducing oxidative stress by chrysin. Journal of Biochemical and Molecular Toxicology, 32(11), e22201.
    1. Aksu, E. H., Kandemir, F. M., Özkaraca, M., Ömür, A. D., Küçükler, S., & Çomaklı, S. (2017). Rutin ameliorates cisplatin-induced reproductive damage via suppression of oxidative stress and apoptosis in adult male rats. Andrologia, 49(1), e12593.

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