Role of the 2-amino group of deoxyguanosine in sequence recognition by EcoRI restriction and modification enzymes
- PMID: 332689
Role of the 2-amino group of deoxyguanosine in sequence recognition by EcoRI restriction and modification enzymes
Abstract
The dG residues within the EcoRI recognition sequence of ColE1 DNA have been selectively replaced with dI. Methylation of the altered sequence by the EcoRI modification enzyme is extremely slow as compared with methyl transfer to the natural recognition site. Since the affinity of the modification enzyme for the dI-containing sequence is considerably less than that for the natural sequence, we have concluded that the 2-amino group of dG has an important role in DNA site recognition by this enzyme. In contrast, the altered site is subject to cleavage by EcoRI endonuclease at rates essentially identical with those observed with the natural sequence. These results strongly suggest that the two enzymes utilize different contacts within the EcoRI site and are consisted with our conclusion (Rubin, R. A., and Modrich, P. (1977) J. Biol. Chem. 252, 7265-7272) that the two proteins interact with their common recognition sequence in different ways.
Similar articles
-
Phenotypically cryptic EcoRI endonuclease activity specified by the ColE1 plasmid.Proc Natl Acad Sci U S A. 1978 Mar;75(3):1265-9. doi: 10.1073/pnas.75.3.1265. Proc Natl Acad Sci U S A. 1978. PMID: 349563 Free PMC article.
-
A dodecapeptide comprising the extended chain-alpha 4 region of the restriction endonuclease EcoRI specifically binds to the EcoRI recognition site.J Biol Chem. 1995 Mar 10;270(10):5122-9. doi: 10.1074/jbc.270.10.5122. J Biol Chem. 1995. PMID: 7890621
-
Role of thymidine residues in DNA recognition by the EcoRI and EcoRV restriction endonucleases.Nucleic Acids Res. 1986 Apr 25;14(8):3463-74. doi: 10.1093/nar/14.8.3463. Nucleic Acids Res. 1986. PMID: 3010238 Free PMC article.
-
How the EcoRI endonuclease recognizes and cleaves DNA.Bioessays. 1992 Jul;14(7):445-54. doi: 10.1002/bies.950140704. Bioessays. 1992. PMID: 1445286 Review.
-
Recognition sequences of restriction endonucleases and methylases--a review.Gene. 1985;33(1):1-102. doi: 10.1016/0378-1119(85)90119-2. Gene. 1985. PMID: 2985469 Review.
Cited by
-
DNA containing the base analogue 2-aminoadenine: preparation, use as hybridization probes and cleavage by restriction endonucleases.Nucleic Acids Res. 1988 Jan 11;16(1):305-17. doi: 10.1093/nar/16.1.305. Nucleic Acids Res. 1988. PMID: 2829119 Free PMC article.
-
Minor-groove-modified oligonucleotides: synthesis of decadeoxynucleotides containing hypoxanthine, N2-methylguanine and 3-deazaadenine, and their interactions with restriction endonucleases Bgl II, Sau, 3AI, and Mbo I (Nucleosides and Nucleotides Part 75).Nucleic Acids Res. 1987 Apr 10;15(7):3059-72. doi: 10.1093/nar/15.7.3059. Nucleic Acids Res. 1987. PMID: 3031618 Free PMC article.
-
Palindromic oligonucleotides containing 7-deaza-2'-deoxyguanosine: solid-phase synthesis of d[(p)GG*AATTCC] octamers and recognition by the endodeoxyribonuclease EcoRI.Nucleic Acids Res. 1986 Mar 11;14(5):2319-32. doi: 10.1093/nar/14.5.2319. Nucleic Acids Res. 1986. PMID: 3008089 Free PMC article.
-
Hydrolysis by restriction endonucleases at their DNA recognition sequences substituted with mismatched base pairs.Nucleic Acids Res. 1986 Jun 11;14(11):4407-20. Nucleic Acids Res. 1986. PMID: 3012472 Free PMC article.
-
KpnI restriction endonuclease and methyltransferase exhibit contrasting mode of sequence recognition.Nucleic Acids Res. 2004 Jun 10;32(10):3148-55. doi: 10.1093/nar/gkh638. Print 2004. Nucleic Acids Res. 2004. PMID: 15192117 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
