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. 2020 Nov 28:2020:10.17912/micropub.biology.000333.
doi: 10.17912/micropub.biology.000333.

The ubiquitylome of developing cortical neurons

Shalini Menon  1 , Dennis Goldfarb  1 , Emily M Cousins  1 , M Ben Major  1 , Stephanie L Gupton  1
Affiliations

The ubiquitylome of developing cortical neurons

Shalini Menon et al. MicroPubl Biol. .
No abstract available

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Figures

Figure 1. The ubiquitylome of developing cortical neurons
Figure 1. The ubiquitylome of developing cortical neurons
A) Graphical representation of approach. Embryonic day 15.5 (E15.5) neurons were dissected from the cortex of mice with indicated genotypes, and cultured for 56 hours prior to addition of proteasomal and deubiquitinase inhibitors for 4 hrs. Half of the plated neurons were treated with 600ng/ml of netrin-1 for 40 min prior to lysis. Neurons were lysed and trypsin digested. Ubiquitylated peptides were enriched and analyzed by mass spectrometry. B-E) Volcano plots demonstrating changes in ubiquitylation of proteins that overlapped with high-confidence TRIM9 and TRIM67 interaction partners between the indicated conditions. F) Pie-chart of biological processes enriched via Gene Ontology (GO) classification of proteins that overlapped between high-confidence TRIM9 and TRIM67 interaction partners and altered DiGly enrichment.
See this image and copyright information in PMC

References

    1. Berti C, Messali S, Ballabio A, Reymond A, Meroni G. TRIM9 is specifically expressed in the embryonic and adult nervous system. Mech Dev. 2002 May 01;113(2):159–162. doi: 10.1016/s0925-4773(02)00013-8. - DOI - PubMed
    1. Boyer NP, McCormick LE, Menon S, Urbina FL, Gupton SL. A pair of E3 ubiquitin ligases compete to regulate filopodial dynamics and axon guidance. J Cell Biol. 2020 Jan 01;219(1) doi: 10.1083/jcb.201902088. - DOI - PMC - PubMed
    1. Boyer NP, Monkiewicz C, Menon S, Moy SS, Gupton SL. Mammalian TRIM67 Functions in Brain Development and Behavior. eNeuro. 2018 Jun 14;5(3) doi: 10.1523/ENEURO.0186-18.2018. - DOI - PMC - PubMed
    1. Fischer DF, van Dijk R, van Tijn P, Hobo B, Verhage MC, van der Schors RC, Li KW, van Minnen J, Hol EM, van Leeuwen FW. Long-term proteasome dysfunction in the mouse brain by expression of aberrant ubiquitin. Neurobiol Aging. 2008 Aug 28;30(6):847–863. doi: 10.1016/j.neurobiolaging.2008.06.009. - DOI - PubMed
    1. Kwiatkowski AV, Rubinson DA, Dent EW, Edward van Veen J, Leslie JD, Zhang J, Mebane LM, Philippar U, Pinheiro EM, Burds AA, Bronson RT, Mori S, Fässler R, Gertler FB. Ena/VASP Is Required for neuritogenesis in the developing cortex. Neuron. 2007 Nov 01;56(3):441–455. doi: 10.1016/j.neuron.2007.09.008. - DOI - PubMed