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. 2021 Mar;595(6):828-843.
doi: 10.1002/1873-3468.14015. Epub 2020 Dec 21.

Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity

Flavia Bieczynski  1   2 , Kathleen Burkhardt-Medicke  2 , Carlos M Luquet  3 , Stefan Scholz  2 , Till Luckenbach  2
Affiliations
Free article

Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity

Flavia Bieczynski et al. FEBS Lett. 2021 Mar.
Free article

Abstract

ATP-binding cassette (ABC) transporter proteins include efflux pumps that confer multixenobiotic resistance to zebrafish embryos, a valuable toxico/pharmacological model. Here, we established an automated microscopy-based rhodamine B dye accumulation assay in which enhanced dye accumulation in live zebrafish embryos indicates inhibition of multixenobiotic efflux transporter activity. Twenty structurally divergent known substrates and/or inhibitors of human ABC transporters and environmentally relevant compounds were examined using this assay and the ATPase activity of recombinant zebrafish Abcb4 as readouts. These two assays confirmed that Abcb4 functions as an efflux transporter in zebrafish, whereas they gave discordant results for some of the tested substances. The dye accumulation assay in zebrafish embryos could be useful to screen environmental pollutants and other chemicals for efflux transporter interaction in a medium-throughput fashion.

Keywords: ABC efflux transporter; ATPase activity; chemosensitizer; dye accumulation assay; zebrafish Abcb4; zebrafish embryo.

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References

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