In Vivo High Plasticity of Multi-Drug Resistant ST258 Klebsiella pneumoniae

Abstract

Carbapenemase production in Enterobacterales clinical isolates is a global threat. Multi-drug resistant Klebsiella pneumoniae harboring carbapenemases are a major concern among the hospital settings in Latin America. Aim: The aim of this study was to analyze the genetic relatedness between three isolates of K. pneumoniae recovered from one patient in the same bacteriological round on the same day, which exhibited different susceptibility profiles to carbapenems (CP) and to colistin (Col). Isolates' profiles were as follows (susceptible-S/resistant-R): CP^S/Col^R, CP^R/Col^R, and CP^R/Col^S. Pulse-field gel electrophoresis, multilocus sequence typing, and whole genome sequencing were performed. Conjugation assays were carried out and PCR determination in transconjugants (Tcs) was made for: bla_CTX-M-groups, bla_NDM, bla_KPC, bla_TEM, qnr alleles, aac(6')Ib-cr, ermB, and plasmid incompatibility groups (Inc). Results: All isolates belonged to the same clone, to ST258 and harbored bla_CTX-M-14, bla_CTX-M-15, qnrA1, qnrB1, aac(6')Ib-cr, and wzi154 (capsule-locus KL107). One isolate had additional wzi gene, wzi109 (capsule-locus KL36). In CP^R isolates, the pattern was explained for bla_NDM-1 or bla_NDM-1/bla_KPC-2 presence, and in Col^R for IS5-like element insertion in mgrB at different positions. Co-mobilization of bla_NDM-1/qnrA1 was associated to a different plasmid Inc (A/C-FII) in both bla_NDM-1 donors. Mobilization of bla_CTX-M-14 was related to IncI1 in one donor. Conclusion: These findings highlight the potential plasticity of ST258 K. pneumoniae clone. To the best of our knowledge, this is the first description of bla_NDM-1/bla_KPC-2-producing K. pneumoniae ST258 in Latin America.

Keywords: Klebsiella pneumoniae; ST258; blaKPC-2; blaNDM-1; plasmid replicons.