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. 2021 Jan:134:104691.
doi: 10.1016/j.jcv.2020.104691. Epub 2020 Nov 18.

Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

F Xavier López-Labrador  1 , Julianne R Brown  2 , Nicole Fischer  3 , Heli Harvala  4 , Sander Van Boheemen  5 , Ondrej Cinek  6 , Arzu Sayiner  7 , Tina Vasehus Madsen  8 , Eeva Auvinen  9 , Verena Kufner  10 , Michael Huber  11 , Christophe Rodriguez  12 , Marcel Jonges  13 , Mario Hönemann  14 , Petri Susi  15 , Hugo Sousa  16 , Paul E Klapper  17 , Alba Pérez-Cataluňa  18 , Marta Hernandez  19 , Richard Molenkamp  20 , Lia van der Hoek  21 , Rob Schuurman  22 , Natacha Couto  23 , Karoline Leuzinger  24 , Peter Simmonds  25 , Martin Beer  26 , Dirk Höper  27 , Sergio Kamminga  28 , Mariet C W Feltkamp  29 , Jesús Rodríguez-Díaz  30 , Els Keyaerts  31 , Xiaohui Chen Nielsen  32 , Elisabeth Puchhammer-Stöckl  33 , Aloys C M Kroes  34 , Javier Buesa  35 , Judy Breuer  36 , Eric C J Claas  37 , Jutte J C de Vries  38 ; ESCV Network on Next-Generation Sequencing
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Free article

Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

F Xavier López-Labrador et al. J Clin Virol. 2021 Jan.
Free article

Abstract

Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.

Keywords: High-throughput sequencing; Next-generation sequencing; Recommendations; Viral metagenomics; Wet lab.

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