An ultra-sensitive and selective AChE based colorimetric detection of malathion using silver nanoparticle-graphene oxide (Ag-GO) nanocomposite

Abstract

Herein, we propose rapid, precise acetylcholinesterase (AChE) inhibition-based sensing strategy for malathion detection in the presence of Ag-GO and acetylthiocholine (ATCh). The biosensing method was developed with a nanocomposite of citrate stabilized AgNPs anchored on the GO sheets (Ag-GO). The physical and chemical properties of the prepared Ag-GO composite were analyzed with various characterization techniques, including XRD, FT-IR, XPS, UV-Visible spectroscopy, and HR-TEM. The positively charged thiocholine (TCh) produced by enzyme hydrolysis triggers the AgNPs aggregation on GO sheets, which ultimately decreases the intensity of the corresponding SPR absorption peak. While the addition of malathion into the sensing system hindered the AChE activity and limited the TCh production, and thus inhibits the decrease in the SPR band intensity. The designed sensing system displayed linearity in the broad range of malathion concentrations (0.01 pM-1000 pM) with a limit of detection and the limit of quantification values of 0.01 pM, and 0.035 pM, respectively. The application of the designed biosensing system was extended to determine the malathion in actual samples namely, tap water, agricultural runoff water, lake water, and grape extract, which resulted in almost 100% recovery rates in all the spiked samples.

Keywords: Acetylcholinesterase; Acetylthiocholine; AgNPs; Graphene oxide; Malathion.