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Review
. 2020 Nov 18:26:e20200019.
doi: 10.1590/1678-9199-JVATITD-2020-0019.

Zika virus serological diagnosis: commercial tests and monoclonal antibodies as tools

Isaura Beatriz Borges Silva  1   2 , Aldacilene Souza da Silva  1 , Mariana Sequetin Cunha  3 , Aline Diniz Cabral  1 , Kelly Cristina Alves de Oliveira  1 , Elizabeth De Gaspari  1   2 , Carlos Roberto Prudencio  1   2
Affiliations
Review

Zika virus serological diagnosis: commercial tests and monoclonal antibodies as tools

Isaura Beatriz Borges Silva et al. J Venom Anim Toxins Incl Trop Dis. .

Abstract

Zika virus (ZIKV), an emerging arthropod-borne virus (arbovirus) of the Flaviviridae family, is a current issue worldwide, particularly because of the congenital and neurological syndromes associated with infection by this virus. As the initial clinical symptoms of all diseases caused by this group are very similar, clinical diagnosis is difficult. Furthermore, laboratory diagnostic efforts have failed to identify specific and accurate tests for each virus of the Flaviviridae family due to the cross-reactivity of these viruses in serum samples. This situation has resulted in underreporting of the diseases caused by flaviviruses. However, many companies developed commercial diagnostic tests after the recent ZIKV outbreak. Moreover, health regulatory agencies have approved different commercial tests to extend the monitoring of ZIKV infections. Considering that a specific and sensitive diagnostic method for estimating risk and evaluating ZIKV propagation is still needed, this review aims to provide an update of the main commercially approved serological diagnostics test by the US Food and Drug Administration (FDA) and Brazilian National Health Surveillance Agency (ANVISA). Additionally, we present the technologies used for monoclonal antibody production as a tool for the development of diagnostic tests and applications of these antibodies in detecting ZIKV infections worldwide.

Keywords: Commercial tests; Flaviviruses; Monoclonal antibody; Serological diagnosis; Zika virus.

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Conflict of interest statement

Competing interests: The authors declare that they have no competing interests.

Figures

Figure 1.
Figure 1.. Schematic representation of an antibody capturing serology method for detection of IgG and IgM antibodies in serum sample using a monoclonal antibody that recognizes ZIKV antigen.
See this image and copyright information in PMC

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