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. 2020 Dec 7;15(12):e0243062.
doi: 10.1371/journal.pone.0243062. eCollection 2020.

Administration of Lactobacillus paracasei HB89 mitigates PM2.5-induced enhancement of inflammation and allergic airway response in murine asthma model

Ching-Hung Lin  1   2 , Chia-Yi Tseng  2   3 , Ming-Wei Chao  1   3
Affiliations

Administration of Lactobacillus paracasei HB89 mitigates PM2.5-induced enhancement of inflammation and allergic airway response in murine asthma model

Ching-Hung Lin et al. PLoS One. .

Abstract

PM2.5 causes abnormal immune response and asthma in animals. In this study, a Balb/c mouse animal model was exposed to PM2.5 to induce asthma. Lactobacillus paracasei HB89 was fed at the same time, in order to observe whether L. paracasei HB89 mitigates respiratory tract allergies stimulated by PM2.5. The results showed that PM2.5 stimulated a significant increase in white blood cells and immunoglobulin (IgE) in OVA-induced allergic Balb/c mice, and IgE in the blood further triggered the release of histamine in the lung immune cells. This not only increased overall immune cell counts, but the lymphocyte counts also increased significantly, resulting in significant inhibitions of cytokines INF-r and TGF-β, and induction of IL-4, IL-5, IL-13 and IL-17a. After feeding with HB89, apart from the absence of observable changes in body weight, the total white blood cell count in the animal blood and IgE response were also be reduced; the proliferation of immune cells in the lungs caused by PM2.5 was slowed down; and histamine and cytokines INF-r and TGF-β were secreted in large quantities, but IL- 4, IL-5, IL-13, IL-17a were inhibited, which effectively reduced the possibility of asthma induction.

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Conflict of interest statement

The authors have read the journal’s policy and have the following competing interests: Yuan Li Tong Co., Ltd. provided support for this study in the form of a grant awarded to MWC. This does not alter our adherence to PLOS ONE policies on sharing data and materials. There are no patents, products in development or marketed products associated with this research to declare.

Figures

Fig 1
Fig 1. Experimental setup of the PM2.5 exposure enhanced mouse model of asthma.
Fig 2
Fig 2. During the period from HB89 pre-feeding, 14 days before the allergy test, through to the allergy testing period (28 days), there was no difference in the weight of the Balb/c mice, other than weight gain over time.
Fig 3
Fig 3
Differences in white blood cells, red blood cells, and hemoglobin in Balb/c mice: A. Red blood cells; B. White blood cells; C. Platelets; ** p <0.0l as compared with the control group.
Fig 4
Fig 4. Differences in anti-OVA IgE concentration expressed in the serum of Balb/c mice; ** p <0.0l as compared with the control group.
Fig 5
Fig 5. Counts of cytokines INF-r, TGF-β, IL-4, IL-5, IL-13 and IL-17a expressed in the serum of Balb/c mice; * and ** are p<0.05 and <0.0l as compared with the control group.
§ and ¶ are p<0.05 as compared with OVA and OVA+HB89 and OVA+PM and OVA+PM+HB89, respectively.
Fig 6
Fig 6. Analysis of immune cells in alveolar lavage fluid of Balb/c mice.
Fig 7
Fig 7
Analysis data for alveolar lavage fluid of Balb/c mice: A. Histamine; B. Differences in expression of cytokines INF-r, TGF-β, IL-4, IL-5, IL-13 and IL-17a. * and ** are p<0.05 and <0.0l as compared with the control group. § and ¶ are p<0.05 as compared with OVA and OVA+HB89 and OVA+PM and OVA+PM+HB89, respectively.
See this image and copyright information in PMC

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