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. 1986;11(1):79-82.
doi: 10.1007/BF00389429.

Transformation of psi- Saccharomyces cerevisiae to psi+ with DNA co-purified with 3 micron circles

H Dai  1 , S H Tsay, P M Lund, B S Cox
Affiliations

Transformation of psi- Saccharomyces cerevisiae to psi+ with DNA co-purified with 3 micron circles

H Dai et al. Curr Genet. 1986.

Abstract

DNA enriched for supercoiled plasmids prepared from the 3 micron plasmid-enriched, [psi+], [2 micron degrees] strain 6-1G-P188 and from the [2 micron+] [psi+] strain LL20 can be used to transform a psi- recipient strain to psi+. Fractionation of the former preparation by electrophoresis showed that the 3 micron plasmid band contained the transforming activity.

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