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. 2020 Dec 2:12:12415-12422.
doi: 10.2147/IJN.S259191. eCollection 2020.

Knockdown of USP8 Inhibits the Growth of Lung Cancer Cells

Zhenhua Rong #  1 , Zongmin Zhu  2 , Shihua Cai  3 , Bingqing Zhang #  4
Affiliations

Knockdown of USP8 Inhibits the Growth of Lung Cancer Cells

Zhenhua Rong et al. Cancer Manag Res. .

Abstract

Purpose: Lung cancer is the deadliest tumor in the world. This study aimed to investigate the effection of USP8 on the proliferation and growth of NSCLC cells.

Methods: The proliferation, migration, invasion, cell cycle progression, and apoptosis of A549 and H1299 cells were evaluated with CCK8, colony formation, scratch, transwell, and flow cytometry experiments. Furthermore, the expression of cell cycle- and apoptosis-related proteins was detected by western blot.

Results: Knockdown of USP8 inhibited the proliferation, migration, invasion, and cell cycle progression of A549 and H1299 cells, and promoted the apoptosis. The results of western blot indicated that knockdown of USP8 down-regulated the expression of Cyclin D1, CDK4, CDK6, p-AKT, and Bcl2, and up-regulated the expression of Bax.

Conclusion: Knockdown of USP8 inhibited the proliferation of human lung cancer cells by regulating cell cycle- and apoptosis-related proteins. USP8 may be a therapeutic target for lung cancer.

Keywords: PI3K/AKT pathway; USP8; apoptosis; lung cancer; proliferation.

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Conflict of interest statement

All authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Knockdown of USP8 inhibited the proliferation of lung cancer cells.
Figure 2
Figure 2
Knockdown of USP8 inhibited the migration and invasion of lung cancer cells.
Figure 3
Figure 3
Knockdown of USP8 inhibited the cell cycle progression of lung cancer cells.
Figure 4
Figure 4
Knockdown of USP8 induced apoptosis of lung cancer cells.
See this image and copyright information in PMC

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