Nucleic Acids Analysis

Yongxi Zhao^#¹, Xiaolei Zuo^#², Qian Li^#³, Feng Chen¹, Yan-Ru Chen⁴, Jinqi Deng⁵, Da Han², Changlong Hao⁶, Fujian Huang⁷, Yanyi Huang⁸, Guoliang Ke⁹, Hua Kuang⁶, Fan Li², Jiang Li^{10

11}, Min Li², Na Li¹², Zhenyu Lin¹³, Dingbin Liu¹⁴, Juewen Liu¹⁵, Libing Liu^{16

17}, Xiaoguo Liu³, Chunhua Lu¹³, Fang Luo¹³, Xiuhai Mao², Jiashu Sun⁵, Bo Tang¹², Fei Wang³, Jianbin Wang¹⁸, Lihua Wang^{10

11}, Shu Wang¹⁵, Lingling Wu², Zai-Sheng Wu⁴, Fan Xia⁷, Chuanlai Xu⁶, Yang Yang², Bi-Feng Yuan¹⁹, Quan Yuan⁹, Chao Zhang², Zhi Zhu²⁰, Chaoyong Yang^{2

20}, Xiao-Bing Zhang⁹, Huanghao Yang¹³, Weihong Tan^{2

9}, Chunhai Fan^{2

3}

Affiliations

¹ Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049 China.
² Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127 China.
³ School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240 China.
⁴ Cancer Metastasis Alert and Prevention Center, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350108 China.
⁵ CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China.
⁶ State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, 214122 China.
⁷ Faculty of Materials Science and Chemistry, Engineering Research Center of Nano-Geomaterials of Ministry of Education, China University of Geosciences, Wuhan, 430074 China.
⁸ College of Chemistry and Molecular Engineering, Biomedical Pioneering Innovation Center (BIOPIC), Beijing Advanced Innovation Center for Genomics (ICG), Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871 China.
⁹ State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 China.
¹⁰ Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800 China.
¹¹ Bioimaging Center, Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210 China.
¹² College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan, 250014 China.
¹³ Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116 China.
¹⁴ College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin, 300071 China.
¹⁵ Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1 Canada.
¹⁶ Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 China.
¹⁷ College of Chemistry, University of Chinese Academy of Sciences, Beijing, 100049 China.
¹⁸ School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Beijing Advanced Innovation Center for Structural Biology (ICSB), Chinese Institute for Brain Research (CIBR), Tsinghua University, Beijing, 100084 China.
¹⁹ Department of Chemistry, Wuhan University, Wuhan, 430072 China.
²⁰ The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 China.

^# Contributed equally.

PMID: 33293939
PMCID: PMC7716629
DOI: 10.1007/s11426-020-9864-7

Review

Nucleic Acids Analysis

Yongxi Zhao et al. Sci China Chem. 2021.

. 2021;64(2):171-203.

doi: 10.1007/s11426-020-9864-7. Epub 2020 Dec 2.

Authors

Affiliations

¹ Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049 China.
² Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127 China.
³ School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240 China.
⁴ Cancer Metastasis Alert and Prevention Center, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350108 China.
⁵ CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China.
⁶ State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, 214122 China.
⁷ Faculty of Materials Science and Chemistry, Engineering Research Center of Nano-Geomaterials of Ministry of Education, China University of Geosciences, Wuhan, 430074 China.
⁸ College of Chemistry and Molecular Engineering, Biomedical Pioneering Innovation Center (BIOPIC), Beijing Advanced Innovation Center for Genomics (ICG), Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871 China.
⁹ State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 China.
¹⁰ Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800 China.
¹¹ Bioimaging Center, Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210 China.
¹² College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan, 250014 China.
¹³ Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116 China.
¹⁴ College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin, 300071 China.
¹⁵ Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1 Canada.
¹⁶ Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 China.
¹⁷ College of Chemistry, University of Chinese Academy of Sciences, Beijing, 100049 China.
¹⁸ School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Beijing Advanced Innovation Center for Structural Biology (ICSB), Chinese Institute for Brain Research (CIBR), Tsinghua University, Beijing, 100084 China.
¹⁹ Department of Chemistry, Wuhan University, Wuhan, 430072 China.
²⁰ The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 China.

^# Contributed equally.

PMID: 33293939
PMCID: PMC7716629
DOI: 10.1007/s11426-020-9864-7

Abstract

Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis. During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs. In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided.

Keywords: DNA nanotechnology; aptamer; biosensing; nucleic acids analysis; signal amplification.

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References

1. Zhao Y, Chen F, Li Q, Wang L, Fan C. Chem Rev. 2015;115:12491–12545. - PubMed
1. Seeman NC, Sleiman HF. Nat Rev Mater. 2018;3:17068.
1. Zheng J, Yang R, Shi M, Wu C, Fang X, Li Y, Li J, Tan W. Chem Soc Rev. 2015;44:3036–3055. - PMC - PubMed
1. Wang X, Chen F, Zhang D, Zhao Y, Wei J, Wang L, Song S, Fan C, Zhao Y. Chem Sci. 2017;8:4764–4770. - PMC - PubMed
1. Duan R, Zuo X, Wang S, Quan X, Chen D, Chen Z, Jiang L, Fan C, Xia F. J Am Chem Soc. 2013;135:4604–4607. - PubMed

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Nucleic Acids Analysis

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Nucleic Acids Analysis

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