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. 2021 Mar 1;60(10):5307-5315.
doi: 10.1002/anie.202014506. Epub 2021 Jan 26.

Simultaneous Dual-Gene Diagnosis of SARS-CoV-2 Based on CRISPR/Cas9-Mediated Lateral Flow Assay

Erhu Xiong  1   2 , Ling Jiang  2 , Tian Tian  1 , Menglu Hu  1 , Huahua Yue  1 , Mengqi Huang  1 , Wei Lin  1 , Yongzhong Jiang  3 , Debin Zhu  4 , Xiaoming Zhou  1
Affiliations

Simultaneous Dual-Gene Diagnosis of SARS-CoV-2 Based on CRISPR/Cas9-Mediated Lateral Flow Assay

Erhu Xiong et al. Angew Chem Int Ed Engl. .

Abstract

Few methods for the detection of SARS-CoV-2 currently have the capability to simultaneously detect two genes in a single test, which is a key measure to improve detection accuracy, as adopted by the gold standard RT-qPCR method. Developed here is a CRISPR/Cas9-mediated triple-line lateral flow assay (TL-LFA) combined with multiplex reverse transcription-recombinase polymerase amplification (RT-RPA) for rapid and simultaneous dual-gene detection of SARS-CoV-2 in a single strip test. This assay is characterized by the detection of envelope (E) and open reading frame 1ab (Orf1ab) genes from cell-cultured SARS-CoV-2 and SARS-CoV-2 viral RNA standards, showing a sensitivity of 100 RNA copies per reaction (25 μL). Furthermore, dual-gene analysis of 64 nasopharyngeal swab samples showed 100 % negative predictive agreement and 97.14 % positive predictive agreement. This platform will provide a more accurate and convenient pathway for diagnosis of COVID-19 or other infectious diseases in low-resource regions.

Keywords: RNA; SARS-CoV-2; genes; polymerases; viruses.

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References

    1. I. Schröder, ACS Chem. Health Saf. 2020, 27, 160-169.
    1. D. Ivanov, Transp. Res. E Logist. Transp. Rev. 2020, 136, 101922-101936.
    1. L. Q. Xia, Acta Laser Biol. Sin. 2020, 29, 1-10.
    1. C. J. Wang, C. Y. Ng, R. H. Brook, JAMA J. Am. Med. Assoc. 2020, 323, 1341-1342.
    1. M. Salathé, C. Althaus, R. Neher, S. Stringhini, E. Hodcroft, J. Fellay, M. Zwahlen, G. Senti, M. Battegay, A. Wilder-Smith, et al., Swiss Med. Wkly. 2020, 150, w202205.

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