Centrosomes in mitotic spindle assembly and orientation

Abstract

The centrosome is present in most animal cells and functions as the major microtubule-organizing center to ensure faithful chromosome segregation during cell division. As cells transition from interphase to mitosis, the duplicated centrosomes separate and move to opposite sides of the cell where the spindle assembles. Centrosomes not only nucleate but also organize microtubules of the mitotic spindle. The mitotic spindle is anchored to the cell cortex by the astral microtubules emanating from the centrosomes. Proper orientation of the mitotic spindle is essential for correct cell division. Centrosome-localized polo-like kinase Plk1 has been linked to regulation of proper spindle orientation. A number of proteins including MISP and NuMA have been implicated in the Plk1-mediated spindle orientation pathway.