Fast removal of toxic hexavalent chromium from an aqueous solution by high-density Geobacter sulfurreducens

Abstract

Hexavalent chromium (Cr(VI)) is a carcinogenic compound that can be removed from contaminated sites by the activity of metal-reducing bacteria. The model bacterium Geobacter sulfurreducens reduces Cr(VI) to less toxic Cr(III) and accumulates Cr ions intracellularly. However, this process is usually slow with small concentrations of Cr(VI) removed in a matter of days. Here, high-density G. sulfurreducens cultures were tested for the capacity to remove Cr(VI) readily. With an initial G. sulfurreducens density of 5.8 × 10⁸ cells ml^-1, 99.0 ± 0.8% of 100 mg l^-1 Cr(VI) was removed after 20 min. With a higher starting Cr(VI) concentration of 200 mg l^-1, G. sulfurreducens with a density of 11.4 × 10⁸ cells ml^-1 removed 99.0 ± 0.4% Cr(VI) after 2 h. Experiments performed with cell-free spent medium indicate that extracellular proteins are major contributors for the reduction of Cr(VI) to Cr(III). Furthermore, results show that most Cr(III) ions ultimately end up inside the bacterial cells where they are less susceptible to re-oxidation. The fast Cr(VI) removal rates observed with high-density G. sulfurreducens demonstrate the potential of this bacterium for bioremediation applications such as the cleaning of industrial wastewaters.

Keywords: Cr(III); Extracellular reduction; Geobacter sulfurreducens; High-density cell population; Toxic Cr(VI).