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Review
. 2021:2222:187-218.
doi: 10.1007/978-1-0716-0997-2_12.

Amplified Fragment Length Polymorphism: Applications and Recent Developments

Thotten Elampilay Sheeja  1   2 , Illathidath Payatatti Vijesh Kumar  3 , Ananduchandra Giridhari  3 , Divakaran Minoo  4 , Muliyar Krishna Rajesh  5 , Kantipudi Nirmal Babu  3
Affiliations
Review

Amplified Fragment Length Polymorphism: Applications and Recent Developments

Thotten Elampilay Sheeja et al. Methods Mol Biol. 2021.

Abstract

AFLP or amplified fragment length polymorphism is a PCR-based molecular technique that uses selective amplification of a subset of digested DNA fragments from any source to generate and compare unique fingerprints of genomes. It is more efficient in terms of time, economy, reproducibility, informativeness, resolution, and sensitivity, compared to other popular DNA markers. Besides, it requires very small quantities of DNA and no prior genome information. This technique is widely used in plants for taxonomy, genetic diversity, phylogenetic analysis, construction of high-resolution genetic maps, and positional cloning of genes, to determine relatedness among cultivars and varietal identity, etc. The review encompasses in detail the various applications of AFLP in plants and the major advantages and disadvantages. The review also considers various modifications of this technique and novel developments in detection of polymorphism. A wet-lab protocol is also provided.

Keywords: AFLP; Epigenetics; Genetic diversity; MSAP; Restriction enzymes; Transcriptomics; cDNA.

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