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. 2021 Apr 1;57(4):2002652.
doi: 10.1183/13993003.02652-2020. Print 2021 Apr.

The respiratory microbiota during and following mechanical ventilation for respiratory infections in children

Emma M de Koff  1   2 , Wing Ho Man  1   3 , Marlies A van Houten  1   4 , Nicolaas J G Jansen  5   6 , Kayleigh Arp  2 , Raiza Hasrat  2 , Elisabeth A M Sanders  2   7 , Debby Bogaert  8   9
Affiliations

The respiratory microbiota during and following mechanical ventilation for respiratory infections in children

Emma M de Koff et al. Eur Respir J. .

Abstract

During mechanical ventilation for an LRTI in children, the respiratory microbiota shifted from Haemophilus- and Moraxella-dominated profiles to profiles dominated by antibiotic-resistant Enterobacteriaceae, and Staphylococcus and Streptococcus species. https://bit.ly/3pGfvhQ

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Conflict of interest statement

Conflict of interest: E.M. de Koff has nothing to disclose. Conflict of interest: W.H. Man has nothing to disclose. Conflict of interest: M.A. van Houten has nothing to disclose. Conflict of interest: N.J.G. Jansen has nothing to disclose. Conflict of interest: K. Arp has nothing to disclose. Conflict of interest: R. Hasrat has nothing to disclose. Conflict of interest: E.A.M. Sanders has nothing to disclose. Conflict of interest: D. Bogaert has nothing to disclose.

Figures

FIGURE 1
FIGURE 1
a–c) Nonmetric multidimensional scaling (NMDS) biplots based on the Bray–Curtis dissimilarity matrix visualising the overall microbiota composition in the a) nasopharynx (NP), b) saliva and c) endotracheal aspirate (ETA) at time of intubation, extubation and after 2 months recovery time, along with eight biomarker operational taxonomic units. Ellipses represent the standard deviation of the data points per subgroup. Alluvial plots of cluster transitions in the d) NP, e) saliva and f) ETA between time of intubation, extubation and after 2 months recovery time. Hierarchical clustering of all samples based on the Bray–Curtis dissimilarity matrix identified seven distinct clusters, characterised by either Streptococcus (1) (STREP 1), Moraxella catarrhalis/nonliquefaciens (MOR), Haemophilus influenzae/haemolyticus (HAEMO), Corynebacterium propinquum/pseudodiphtheriticum with Dolosigranulum pigrum (COR/DOL), Streptococcus salivarius (7) (STREP 2), Enterobacter/Klebsiella (ENTERO), or Staphylococcus aureus/epidermidis (STAPH). Stacked bars represent the number of samples in each cluster per time point, and connections between bars represent transitions of participants with two consecutive samples available between time points.
See this image and copyright information in PMC

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