Evaluation of some of the parameters of the enzyme-linked immunospecific assay

Abstract

In an attempt to standardize the procedure for the enzyme-linked immunospecific assay, several parameters were examined. It was determined that horseradish peroxidase was the enzyme of choice and that several brands of polystyrene tubes and plates could be used. The test was considerably shortened by omitting long incubation steps previously used for eliminating background fixation of conjugate. The reproducibility of the procedure proved to be excellent, but reactivity curves indicated that use of a single dilution is not adequate for quantitative tests. A "standard" procedure was proposed.