Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Dec 11;15(12):e0243632.
doi: 10.1371/journal.pone.0243632. eCollection 2020.

Anti-inflammatory, wound healing and antioxidant potential of compounds from Dioscorea bulbifera L. bulbils

Prapaporn Chaniad  1 , Supinya Tewtrakul  2 , Teeratad Sudsai  2 , Supat Langyanai  2 , Kantarakorn Kaewdana  1
Affiliations

Anti-inflammatory, wound healing and antioxidant potential of compounds from Dioscorea bulbifera L. bulbils

Prapaporn Chaniad et al. PLoS One. .

Abstract

Background: Dioscorea bulbifera L. (Dioscoreaceae) has been traditionally used in Thai folk medicine as a diuretic and anthelmintic, for longevity preparations, and for wound and inflammation treatment. This plant is also commonly used in traditional Indian and Chinese medicines in the treatment of sore throat, gastric cancer, rectal carcinoma and goiters. However, the wound healing effects of the active compounds in this plant have not been investigated.

Objective: This study aimed to identify compounds responsible for the wound healing activity of D. bulbifera and determine their potential anti-inflammatory and antioxidant activities.

Methods: Crude extracts of D. bulbifera bulbils, their derived fractions and eleven purified compounds were tested for anti-inflammatory activity against LPS-induced NO production in RAW264.7 macrophages. The wound healing effects were evaluated via cell proliferation and migration assays using human dermal fibroblasts (HDFs), and the antioxidant effects were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical (•OH) scavenging activity assays.

Results: 15,16-Epoxy-6α-O-acetyl-8β-hydroxy-19-nor-clero-13(16),14-diene-17,12;18,2-diolide (2), (+)-catechin (5), quercetin (6) and myricetin (11) exhibited significantly potent wound healing effects and promoted marked cell proliferation, resulting in % viabilities of 107.4-137.6, 121.1-151.9, 98.0-131.9, 90.9-115.9, respectively. Among them, (+)-catechin produced the highest % cell migration, resulting in 100.0% wound closure sooner (at day 2) than the other compounds. In addition, 1 μg/ml (+)-catechin significantly increased fibroblast migration by 2.4-fold compared to that in the control after 24 h. Regarding anti-inflammatory properties, kaempferol (7) and quercetin (6) decreased (p < 0.005) NO production, with IC50 values of 46.6 and 56.2 μM, respectively. In addition, the crude extracts, solvent fractions and flavonoid compounds were also found to possess marked antioxidant activity in both DPPH and •OH radical scavenging assays.

Conclusions: These findings provide more evidence to support the traditional use of D. bulbifera for the treatment of wounds and inflammation.

PubMed Disclaimer

Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Structures of the compounds isolated from bulbils of D. bulbifera.
Fig 2
Fig 2. Effect of the CHCl3, EtOAc, water fractions and A. vera gel on HDF migration.
Images were captured at day 0 and showed that an artificial wounded monolayer was created using the scratch assay. Then, treatments were applied with 1 and 3 μg/ml CHCl3 fraction, 1 and 3 μg/ml EtOAc fraction, 3 and 10 μg/ml water fraction, 1 and 3 μg/ml A. vera gel and control without treatment. Another set of images of fibroblast cell migration were captured at day 1, 2 and 3 after incubation. The cleared areas represented wound and shaded areas resulting in cell migration, which represents the wound closure.
Fig 3
Fig 3. Effect of compounds 2, 5, 6, 11 and A. vera gel on HDF migration.
Images were captured at day 0 and showed that an artificial wounded monolayer was created using the scratch assay. Then treated with 3 and 10 μM 15,16-epoxy-6α-O-acetyl-8β-hydroxy-19-nor-clero-13(16),14-diene-17,12,18,2-diolide (2), 1 and 3 μM (+)-catechin (5), 1 and 3 μM quercetin (6), 1 and 3 μM myricetin (11), 1 and 3 μg/ml A. vera gel and control without treatment. Another set of images of fibroblast cell migration were captured at day 1, 2 and 3 after incubation. The cleared areas represented wound and shaded areas resulting in cell migration, which represents the wound closure.
See this image and copyright information in PMC

References

    1. Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2017;9(6):7204–18. 10.18632/oncotarget.23208 - DOI - PMC - PubMed
    1. Nagaraja S, Wallqvist A, Reifman J, Mitrophanov AY. Computational approach to characterize causative factors and molecular indicators of chronic wound inflammation. J Immunol. 2014;192(4):1824 10.4049/jimmunol.1302481 - DOI - PubMed
    1. Harper D, Young A, McNaught C-E. The physiology of wound healing. Surgery (Oxford). 2014;32(9):445–50.
    1. Sorg H, Tilkorn DJ, Hager S, Hauser J, Mirastschijski U. Skin wound healing: an update on the current knowledge and concepts. Eur Surg Res. 2017;58(1–2):81–94. 10.1159/000454919 - DOI - PubMed
    1. Järbrink K, Ni G, Sönnergren H, Schmidtchen A, Pang C, Bajpai R, et al. Prevalence and incidence of chronic wounds and related complications: a protocol for a systematic review. Syst Rev. 2016;5(1):152 10.1186/s13643-016-0329-y - DOI - PMC - PubMed

Publication types