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Comment
. 2020 Dec 10;183(6):1464-1466.
doi: 10.1016/j.cell.2020.11.022.

Cas9 in Human Embryos: On Target but No Repair

Eva R Hoffmann  1 , Ignasi Roig  2
Affiliations
Free article
Comment

Cas9 in Human Embryos: On Target but No Repair

Eva R Hoffmann et al. Cell. .
Free article

Abstract

In this issue of Cell, Zuccaro and colleagues show that on-target Cas9-mediated double-strand breaks cause chromosome loss or mis-repair of the disease allele in > 90% of human embryos. End joining repair pathways dominate, causing small insertions or deletions, which raises serious questions about using double-strand breaks for "gene surgery".

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  • Allele-Specific Chromosome Removal after Cas9 Cleavage in Human Embryos.
    Zuccaro MV, Xu J, Mitchell C, Marin D, Zimmerman R, Rana B, Weinstein E, King RT, Palmerola KL, Smith ME, Tsang SH, Goland R, Jasin M, Lobo R, Treff N, Egli D. Zuccaro MV, et al. Cell. 2020 Dec 10;183(6):1650-1664.e15. doi: 10.1016/j.cell.2020.10.025. Epub 2020 Oct 29. Cell. 2020. PMID: 33125898

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