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. 2021 Jan;70(1):21-28.
doi: 10.2144/btn-2020-0073. Epub 2020 Dec 14.

tANCHOR: a novel mammalian cell surface peptide display system

Daniel Ivanusic  1 , Kazimierz Madela  2 , Heidi Burghard  1 , Gudrun Holland  2 , Michael Laue  2 , Norbert Bannert  1
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Free article

tANCHOR: a novel mammalian cell surface peptide display system

Daniel Ivanusic et al. Biotechniques. 2021 Jan.
Free article

Abstract

A novel tool for the presentation of peptides and small proteins on the surface of human cells has been developed. Our tANCHOR system utilizes tetraspanin anchors containing heterologous amino acid sequences inserted instead of the large extracellular loop. This technology allows a highly effective extracellular display of epitopes for antibody binding studies and many other potential applications.

Keywords: 2F5; CD20; LEL; PD-1; antibodies; display system; large extracellular loop; plasma membrane; tetraspanin anchors.

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