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. 2021 Feb 17;65(3):e01193-20.
doi: 10.1128/AAC.01193-20. Print 2021 Feb 17.

BKC-2, a New BKC Variant Detected in MCR-9.1-Producing Enterobacter hormaechei subsp. xiangfangensis

Willames M B S Martins #  1   2 , Evelin R Martins #  3 , Letícia K de Andrade  3 , Refath Farzana  2 , Timothy R Walsh  4 , Mark A Toleman  2 , Mara C L Nogueira  5 , Ana C Gales  1
Affiliations

BKC-2, a New BKC Variant Detected in MCR-9.1-Producing Enterobacter hormaechei subsp. xiangfangensis

Willames M B S Martins et al. Antimicrob Agents Chemother. .

Abstract

We characterized a multidrug-resistant (MDR) Enterobacter spp. isolate highlighting the genetic aspects of the antimicrobial resistance genes. An Enterobacter spp. isolate (Ec61) was recovered in 2014 from a transtracheal aspirate sample from a patient admitted to a Brazilian tertiary hospital and submitted to further microbiological and genomic characterization. Ec61 was identified as Enterobacter hormaechei subsp. xiangfangensis strain ST451, showing an MDR profile and the presence of genes codifying the new β-lactamase variants BKC-2 and ACT-84 and the mobile colistin resistance gene mcr-9.1.

Keywords: antimicrobial resistance; carbapenemase; phosphoethanolamine transferase; plasmids.

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Figures

FIG 1
FIG 1
(A) Graphic map of the three plasmids detected in E. hormaechei subsp. xiangfangensis carrying blaBKC-2 and mcr-9.1. (B) Colinear analysis of the two different plasmids harboring blaBKC. Arrows, transcription direction of genes and ORFs. Genes were grouped according to their predictive functions, indicated by color.
FIG 2
FIG 2
(A) Circular comparison between pEc61A and 13 plasmids carrying mcr-9.1 belonging to IncH2 and IncH2a. Pink arrows, conserved regions, including mer operon, nickel resistance module, and tellurium resistance operon. (B) Circular comparison of the six best hit plasmids related to pEc61A on BLASTN analysis of NCBI database. Five of six analyzed plasmids belonged to the IncFIB group, and only p1_045523 also harbored mcr-9. GenBank accession numbers for each plasmid are included in Table S2 in the supplemental material.
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References

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