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. 2021:2254:259-272.
doi: 10.1007/978-1-0716-1158-6_16.

M(R)apping RNA-Protein Interactions

Jasmine Barra  1   2 , Roberto Vendramin  1   2 , Eleonora Leucci  3
Affiliations

M(R)apping RNA-Protein Interactions

Jasmine Barra et al. Methods Mol Biol. 2021.

Abstract

The systematic investigation of RNA-protein interactions is a key step towards a better understanding of the functions of RNA molecules.We developed an easy-to-use method to isolate and identify RNAs and proteins bound to long non-coding RNAs (lncRNAs ) in their native configuration. Similar to other methodologies, we utilize biotinylated antisense oligonucleotides (ASOs) to purify the lncRNA of interest and its associated proteins from different cellular compartments.

Keywords: Mass spectrometry; Proteins; RAP; Subcellular fractions; lncRNAs.

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References

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