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. 2020 Nov 1;7(1):175.
doi: 10.1038/s41438-020-00396-9.

Fine mapping and identification of candidate genes for the peach powdery mildew resistance gene Vr3

Neus Marimon  1   2   3 , Jordi Luque  3 , Pere Arús  1   2 , Iban Eduardo  4   5
Affiliations

Fine mapping and identification of candidate genes for the peach powdery mildew resistance gene Vr3

Neus Marimon et al. Hortic Res. .

Abstract

Powdery mildew is one of the major diseases of peach (Prunus persica), caused by the ascomycete Podosphaera pannosa. Currently, it is controlled through calendar-based fungicide treatments starting at petal fall, but an alternative is to develop resistant peach varieties. Previous studies mapped a resistance gene (Vr3) in interspecific populations between almond ('Texas') and peach ('Earlygold'). To obtain molecular markers highly linked to Vr3 and to reduce the number of candidate genes, we fine-mapped Vr3 to a genomic region of 270 kb with 27 annotated genes. To find evidence supporting one of these positional candidate genes as being responsible of Vr3, we analyzed the polymorphisms of the resequences of both parents and used near-isogenic lines (NILs) for expression analysis of the positional candidate genes in symptomatic or asymptomatic leaves. Genes differentially expressed between resistant and susceptible individuals were annotated as a Disease Resistance Protein RGA2 (Prupe2G111700) or an Eceriferum 1 protein involved in epicuticular wax biosynthesis (Prupe2G112800). Only Prupe2G111700 contained a variant predicted to have a disruptive effect on the encoded protein, and was overexpressed in both heterozygous and homozygous individuals containing the Vr3 almond allele, compared with susceptible individuals. This information was also useful to identify and validate molecular markers tightly linked and flanking Vr3. In addition, the NILs used in this work will facilitate the introgression of this gene into peach elite materials, alone or pyramided with other known resistance genes such as peach powdery mildew resistance gene Vr2.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1. Relative normalized expression of candidate genes with significant differences in symptomatic and asymptomatic leaves (p < 0.01).
Solid and dashed lines correspond to homozygous (Vr3Vr3) and heterozygous (Vr3vr3) individuals for the Vr3 allele from ‘Texas’, respectively. Dotted lines correspond to individuals with Vr3 peach alleles. Bars indicate standard error of the mean
See this image and copyright information in PMC

References

    1. Arús P, Verde I, Sosinski B, Zhebentyayeva T, Abbott AG. The peach genome. Tree Genet. Genomes. 2012;8:531–547. doi: 10.1007/s11295-012-0493-8. - DOI
    1. Verde I, et al. The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity, domestication and genome evolution. Nat. Genet. 2013;45:487–494. doi: 10.1038/ng.2586. - DOI - PubMed
    1. Peace C. DNA-informed breeding of rosaceous crops: promises, progress and prospects. Hortic. Res. 2017;4:17006. doi: 10.1038/hortres.2017.6. - DOI - PMC - PubMed
    1. FAOSTAT. FAO Corporate statistical database. Food and Agriculture Organization of the United Nations. http://www.fao.org/faostat/en/#data/QC (2018).
    1. Pascal T, Pfeiffer F, Kervella J. Powdery mildew resistance in the peach cultivar Pamirskij 5 is genetically linked with the Gr gene for leaf color. HortScience. 2010;45:150–152. doi: 10.21273/HORTSCI.45.1.150. - DOI