Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Sep;32(5):767-779.
doi: 10.1002/pca.3022. Epub 2020 Dec 17.

The composition differences between small black beans and big black beans from different habitats and its effects on the processing of Polygonum multiflorum

Wanning Chen  1 , Piao Wang  1 , Hongxi Chen  1 , Yanchao Xing  1 , Caixiang Liu  2 , Guixiang Pan  3 , Zhiying Dou  1 , Lifeng Han  1
Affiliations

The composition differences between small black beans and big black beans from different habitats and its effects on the processing of Polygonum multiflorum

Wanning Chen et al. Phytochem Anal. 2021 Sep.

Abstract

Introduction: The roots of Polygonum multiflorum (PM) serve as a classical traditional Chinese medicine (TCM), which has multiple biological activities. However, many cases of hepatotoxicity in PM have been reported in recent years. Processing PM with black beans decoction is one of the typical processing methods to reduce the hepatotoxicity of PM since ancient times.

Objectives: To find potential effective constituents, as well as the optimal variety and origin of black beans for the processing of PM.

Methods: Based on ultrahigh-performance liquid chromatography Q-Orbitrap mass spectrometry (UHPLC-Q-Orbitrap-MS) analysis, we measured the contents of the two potential toxic compounds (emodin-8-O-glucoside and torachrysone-O-hexose) in raw PM (R-PM), PM processed with big black beans (B-PM) and PM processed with small black beans (S-PM). The flow cytometry method analysed the effects of different processed products of PM on apoptosis of L02 cells in different drug concentration. Proton nuclear magnetic resonance (1 H-NMR) and UHPLC-Q-Orbitrap-MS together with multivariate statistical analysis were used to systematically analyse the different components between small black beans (Small-BB) and big black beans (Big-BB) from 30 different habitats.

Results: The toxicity was ranked from small to large: S-PM < B-PM < R-PM. Processing PM with black beans could significantly decrease the apoptosis rate of L02 cells, especially when the drug concentration is 80 μg/mL. Besides, we find five differential compounds (α-arabinose, α-galactose, proline, isomer of daidzein and isomer of genistein) may be potential active ingredients. In terms of the black beans collected from 30 producing areas, we find that Small-BB from Weifang in Shandong province was optimum to processing PM, followed by Shangqiu in Henan province, Jilin and Liaoning province.

Conclusion: The ingredients that affect the processing of PM may be attributed to α-arabinose, α-galactose, proline, isomer of daidzein and isomer of genistein in black beans. When the drug concentration is higher, the effect of reducing the hepatotoxicity of PM is better. Besides, Small-BB was more effective than Big-BB for reducing the toxicity of PM, especially Small-BB from Weifang in Shandong, Shangqiu in Henan province and northeast China.

Keywords: 1H-NMR; Polygonum multiflorum; UHPLC-Q-Orbitrap-MS; big black beans; hepatotoxicity; metabolomics; small black beans.

PubMed Disclaimer

References

REFERENCES

    1. Yan Y, Shi N, Han XY, Li GD, Wen BY, Gao J. UPLC/MS/MS-based metabolomics study of the hepatotoxicity and nephrotoxicity in rats induced by Polygonum multiflorum Thunb. ACS Omega. 2020;5(18):10489-10500.
    1. Lin L, Lin H, Zhang M, et al. A novel method to analyze hepatotoxic components in Polygonum multiflorum using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. J Hazard Mater. 2015;299:249-259.
    1. Kim YJ, Lee JY, Kin HJ, Kim DH, Lee TH. Inhibitory effect of EMODIN on raw 264.7 activated with double stranded RNA analogue poly I:c. Afr J Tradit Complement Altern Med. 2017;14(3):157-166.
    1. Lv LS, Cheng YH, Zheng TS, Li XM, Zhai R. Purification, antioxidant activity and antiglycation of polysaccharides from Polygonum multiflorum Thunb. Carbohyd Polym. 2014;99:765-773.
    1. Chin YT, Cheng GY, Shih YJ, et al. Therapeutic applications of resveratrol and its derivatives on periodontitis. Ann NY Acad Sci. 2017;1403(1):101-108.

LinkOut - more resources