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. 1977 Jul;4(7):2389-406.
doi: 10.1093/nar/4.7.2389.

Molecular cloning of extensive sequences of the in vitro synthesized chicken ovalbumin structural gene

P Humphries, M Cochet, A Krust, P Gerlinger, P Kourilsky, P Chambon
Free PMC article

Molecular cloning of extensive sequences of the in vitro synthesized chicken ovalbumin structural gene

P Humphries et al. Nucleic Acids Res. 1977 Jul.
Free PMC article

Abstract

Double-stranded DNA molecules complementary to ovalbumin chicken messenger RNA were synthesized in vitro and integrated into the E. coli plasmid pCR1 using an oligodG-dc tailing procedure. The resultant hybrid plasmids, amplified by transfection of E. coli, were shown by hybridization and gel electrophoresis to contain extensive DNA sequences of the ovalbumin structural gene.

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