doi: 10.1093/nar/4.8.2767.
Modified polynucleotides. I. Investigation of the enzymatic polymerization of 5-alkyl-dUTP-s
- PMID: 333394
- PMCID: PMC342607
- DOI: 10.1093/nar/4.8.2767
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Modified polynucleotides. I. Investigation of the enzymatic polymerization of 5-alkyl-dUTP-s
Nucleic Acids Res.
1977 Aug.
Free PMC article
Abstract
The chemical synthesis of 5-alkyl-dUTP-s and their participation as substrates in poly[d(A-6)] primed polymerization reactions with dATP by E. coli DNA polymerase I enzyme has been described. In comparison with dTTP, at saturating substrate concentrations, the rate of hypochromic effect was found to be 17.3% higher for dUTP and was lower by 27.4% for 5-ethyl-dUTP, 29.5% for 5-n-propyl-dUTP, 31.4% for 5-n-butyl-dUTP and by 85.0% for 5-n-pentyl-dUTP. No hypochromic effect could be observed, however, with 5-iso-propyl-, 5-tert.butyl- and 5-n-hexyl-dUTP-s. Polydeoxynucleotides have also been isolated from the reaction mixture and some of their structural properties determined.
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