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Review
. 2021 Jul 5;15(7):1222-1235.
doi: 10.1093/ecco-jcc/jjaa257.

Organoid-based Models to Study the Role of Host-microbiota Interactions in IBD

Martina Poletti  1   2 , Kaline Arnauts  3   4 , Marc Ferrante  3   5 , Tamas Korcsmaros  1   2
Affiliations
Review

Organoid-based Models to Study the Role of Host-microbiota Interactions in IBD

Martina Poletti et al. J Crohns Colitis. .

Erratum in

  • Correction.
    [No authors listed] [No authors listed] J Crohns Colitis. 2023 Jan 27;17(1):149. doi: 10.1093/ecco-jcc/jjac104. J Crohns Colitis. 2023. PMID: 35971821 Free PMC article. No abstract available.

Abstract

The gut microbiota appears to play a central role in health, and alterations in the gut microbiota are observed in both forms of inflammatory bowel disease [IBD], namely Crohn's disease and ulcerative colitis. Yet, the mechanisms behind host-microbiota interactions in IBD, especially at the intestinal epithelial cell level, are not yet fully understood. Dissecting the role of host-microbiota interactions in disease onset and progression is pivotal, and requires representative models mimicking the gastrointestinal ecosystem, including the intestinal epithelium, the gut microbiota, and immune cells. New advancements in organoid microfluidics technology are facilitating the study of IBD-related microbial-epithelial cross-talk, and the discovery of novel microbial therapies. Here, we review different organoid-based ex vivo models that are currently available, and benchmark their suitability and limitations for specific research questions. Organoid applications, such as patient-derived organoid biobanks for microbial screening and 'omics technologies, are discussed, highlighting their potential to gain better mechanistic insights into disease mechanisms and eventually allow personalised medicine.

Keywords: in vitro models; Inflammatory bowel disease; microbiota; organoids.

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Figures

Figure 1.
Figure 1.
[A] The intestinal epithelium and the organoid model. [B] 3D organoids and organoid-derived models. [C] Microfluidics models.
Figure 2.
Figure 2.
Organoid-based models, [A] evolution; [B] and applications.
See this image and copyright information in PMC

Comment in

  • Letter to the Editor.
    ECCO Governing Board. ECCO Governing Board. J Crohns Colitis. 2022 Nov 23;16(11):1792-1793. doi: 10.1093/ecco-jcc/jjab225. J Crohns Colitis. 2022. PMID: 35073577 No abstract available.

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