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. 1977 Aug;74(8):3259-63.
doi: 10.1073/pnas.74.8.3259.

Packaging recombinant DNA molecules into bacteriophage particles in vitro

B Hohn, K Murray

Packaging recombinant DNA molecules into bacteriophage particles in vitro

B Hohn et al. Proc Natl Acad Sci U S A. 1977 Aug.

Abstract

Recombinant phage genomes made in reactions with purified enzymes may be recovered directly by packaging into phage heads in vitro. The process is efficient and nonselective and offers containment in initial stages of handling recombinant DNA. Ligase [poly(deoxyribonucleotide):poly-(deoxyribonucleotide) ligase (AMP-forming), EC 6.5.1.1] reaction products can recombine with endogenous phage DNA during packaging, but UV-irradiation eliminates the biological activity of the endogenous DNA.

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