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. 2020 Dec;17(4):236-239.
doi: 10.4274/tjod.galenos.2020.90359. Epub 2020 Dec 10.

Endometrial CD56+ natural killer cells in women with recurrent implantation failure: An immunohistochemical study

Gulchin Babayeva  1 , Yunus Emre Purut  2 , Burak Giray  2 , Pembe Oltulu  3 , Rabia Alakuş  3 , Mehmet Cengiz Çolakoğlu  1
Affiliations

Endometrial CD56+ natural killer cells in women with recurrent implantation failure: An immunohistochemical study

Gulchin Babayeva et al. Turk J Obstet Gynecol. 2020 Dec.

Abstract

Objective: Implantation failure is a multifactorial problem of reproductive medicine. However, the mechanism of this process is still not fully understood. There is increasing evidence that these cases of recurrent implantation failure might have an immunologic background. Uterine natural killer (NK) cells provide immune-modulation at the interface between maternal decidua and the trophoblast. The aim of this study to evaluate whether there was a significant difference in the number of endometrial CD56+ NK between women with a history of recurrent implantation failure and women who had a live birth.

Materials and methods: Patients with a history of recurrent implantation failure were included in the study. Twenty-five women with a history of recurrent implantation failure were assigned to the case group, and 25 women who had one or more live births were assigned to the control group. Endometrial biopsies were obtained during the luteal phase on the 21st-24th day of the menstrual cycle.

Results: There was a statistically significant difference between the groups concerning the number of deliveries (p<0.001) and miscarriages (p<0.001). The mean number of uNK was 10.5±10.5 cells/mm2 in the case group and 19.2±11.2 cells/mm2 in the control group. There was a statistically significant difference between the two groups (p=0.003).

Conclusion: Implantation failure is a multifactorial problem of reproductive medicine. The results of our study suggest that uterine NK play a role in the progress of normal pregnancy and reduced uterine NK cell numbers were associated with implantation failure.

Keywords: CD56; immunohistochemistry; in vitro fertilization; recurrent implantation failure; uterine natural killer cells.

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Conflict of interest statement

Conflict of Interest: The authors report no conflict of interest.

Figures

Figure 1
Figure 1
CD56+ cells were shown in the endometrial tissue
See this image and copyright information in PMC

References

    1. Coughlan C, Ledger W, Wang Q, Liu F, Demirol A, Gurgan T, et al. Recurrent implantation failure: definition and management. Reprod Biomed Online. 2014;28:14–38. - PubMed
    1. Van Mourik MS, Macklon NS, Heijnen CJ. Embryonic implantation: cytokines, adhesion molecules, and immune cells in establishing an implantation environment. J Leukoc Biol. 2009;85:4–19. - PubMed
    1. Blois S, Tometten M, Kandil J, Hagen E, Klapp BF, Margni RA, et al. Intercellular adhesion molecule-1/LFA-1 cross talk is a proximate mediator capable of disrupting immune integration and tolerance mechanism at the feto-maternal interface in murine pregnancies. J Immunol. 2005;174:1820–9. - PubMed
    1. Koopman LA, Kopcow HD, Rybalov B, Boyson JE, Orange JS, Schatz F, et al. Human decidual natural killer cells are a unique NK cell subset with immunomodulatory potential. J Exp Med. 2003;198:1201–12. - PMC - PubMed
    1. Nagler A, Lanier L, Cwirla S, Phillips J. Comparative studies of human FcRIII-positive and negative natural killer cells. J Immunol. 1989;143:3183–91. - PubMed

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