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. 2021 Dec;10(1):1-20.
doi: 10.1080/21623945.2020.1859789.

Mouse Embryonic Fibroblast Adipogenic Potential: A Comprehensive Transcriptome Analysis

Mohamed Al-Sayegh  1 , Hamad Ali  2   3 , Mohammad H Jamal  4 , Mei ElGindi  1 , Tina Chanyong  1 , Khulood Al-Awadi  5 , Mohamed Abu-Farha  6
Affiliations

Mouse Embryonic Fibroblast Adipogenic Potential: A Comprehensive Transcriptome Analysis

Mohamed Al-Sayegh et al. Adipocyte. 2021 Dec.

Abstract

Our understanding of adipose tissue has progressed from an inert tissue for energy storage to be one of the largest endocrine organs regulating metabolic homoeostasis through its ability to synthesize and release various adipokines that regulate a myriad of pathways. The field of adipose tissue biology is growing due to this association with various chronic metabolic diseases. An important process in the regulation of adipose tissue biology is adipogenesis, which is the formation of new adipocytes. Investigating adipogenesis in vitro is currently a focus for identifying factors that might be utilized in clinically. A powerful tool for such work is high-throughput sequencing which can rapidly identify changes at gene expression level. Various cell models exist for studying adipogenesis and has been used in high-throughput studies, yet little is known about transcriptome profile that underlies adipogenesis in mouse embryonic fibroblasts. This study utilizes RNA-sequencing and computational analysis with DESeq2, gene ontology, protein-protein networks, and robust rank analysis to understand adipogenesis in mouse embryonic fibroblasts in-depth. Our analyses confirmed the requirement of mitotic clonal expansion prior to adipogenesis in this cell model and highlight the role of Cebpa and Cebpb in regulating adipogenesis through interactions of large numbers of genes.

Keywords: Adipose tissue; adipocyte differentiation; adipogenesis; mouse embryonic fibroblast; transcriptome.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1.
Figure 1.
MEF capability to acquiring adipogenic features
Figure 2.
Figure 2.
Correlation analysis and number of differentially expressed genes
Figure 3.
Figure 3.
Differentially expressed genes (DEGs) GO term and PPI analysis
Figure 4.
Figure 4.
Degrees of centrality and GO term of DEGs
Figure 5.
Figure 5.
Verification of Cebpa and Cebpb gene expression and their association to other gene networks
Figure 6.
Figure 6.
PPI network of Cebpa and Cebpb in relation to identified DEGs
See this image and copyright information in PMC

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