Distinct Preflowering Drought Tolerance Strategies of Sorghum bicolor Genotype RTx430 Revealed by Subcellular Protein Profiling
- PMID: 33352693
- PMCID: PMC7767018
- DOI: 10.3390/ijms21249706
Distinct Preflowering Drought Tolerance Strategies of Sorghum bicolor Genotype RTx430 Revealed by Subcellular Protein Profiling
Abstract
Drought is the largest stress affecting agricultural crops, resulting in substantial reductions in yield. Plant adaptation to water stress is a complex trait involving changes in hormone signaling, physiology, and morphology. Sorghum (Sorghum bicolor (L.) Moench) is a C4 cereal grass; it is an agricultural staple, and it is particularly drought-tolerant. To better understand drought adaptation strategies, we compared the cytosolic- and organelle-enriched protein profiles of leaves from two Sorghum bicolor genotypes, RTx430 and BTx642, with differing preflowering drought tolerances after 8 weeks of growth under water limitation in the field. In agreement with previous findings, we observed significant drought-induced changes in the abundance of multiple heat shock proteins and dehydrins in both genotypes. Interestingly, our data suggest a larger genotype-specific drought response in protein profiles of organelles, while cytosolic responses are largely similar between genotypes. Organelle-enriched proteins whose abundance significantly changed exclusively in the preflowering drought-tolerant genotype RTx430 upon drought stress suggest multiple mechanisms of drought tolerance. These include an RTx430-specific change in proteins associated with ABA metabolism and signal transduction, Rubisco activation, reactive oxygen species scavenging, flowering time regulation, and epicuticular wax production. We discuss the current understanding of these processes in relation to drought tolerance and their potential implications.
Keywords: FLOWERING LOCUS C; abiotic stress; abscisic acid; adaptive mechanisms; drought stress; drought tolerance; postflowering; preflowering; proteomics; reactive oxygen species; rubisco activase; sorghum.
Conflict of interest statement
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
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References
-
- Kuwayama Y., Thompson A., Bernknopf R., Zaitchik B., Vail P. Estimating the Impact of Drought on Agriculture Using the U.S. Drought Monitor. Am. J. Agric. Econ. 2018;101:193–210. doi: 10.1093/ajae/aay037. - DOI
-
- United Nations, Department of Economic and Social Affairs . World Population Prospects Highlights. 2019. [(accessed on 15 October 2020)]. Available online: https://www.un.org/development/desa/publications/world-population-prospe....
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