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. 2020 Nov 26;9(1):1340.
doi: 10.4102/ajlm.v9i1.1340. eCollection 2020.

Will long-read sequencing technologies replace short-read sequencing technologies in the next 10 years?

Boluwatife A Adewale  1   2   3
Affiliations

Will long-read sequencing technologies replace short-read sequencing technologies in the next 10 years?

Boluwatife A Adewale. Afr J Lab Med. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

The author declares that no competing interests exist.

Figures

FIGURE 1
FIGURE 1
Overview of short- and long-read sequencing technologies. Short-read sequencing methods are displayed on the left, long-read sequencing methods are shown on the right. Short-read sequencing methods are classified into sequencing by synthesis and sequencing by ligation. They all require clonal amplification; emulsion polymerase chain reaction for 454 pyrosequencing, SOLiD™ and Ion Torrent, SSBA is peculiar to Illumina, solid-phase template walking is used for certain SOLiD™ technologies. Illumina, fluorescent-labelled dNTP added to bridge amplified DNA template; 454 pyrosequencing, empolymerase chain reaction-generated microbead-bound DNA clone in picotitre well, DNA polymerase is added to the well, nucleotides are washed over in turn, deoxynucleoside triphosphate incorporation monitored via pyrophosphate release; Ion Torrent, similar to 454 pyrosequencing, deoxynucleoside triphosphate incorporation monitored via H+ ions release detected by a pH sensor that uses complementary metal-oxide-semiconductor technology. SOLiD™, microbead-bound DNA template flanked by adapters is hybridized to a growing complementary strand, under the action of DNA ligase. cPAL, another sequencing by ligation technique not described in this paper, employed by Complete Genomics, anchor sequence and probes hybridize to DNA template in a series of ligation reactions taking place on a nanoball. PacBio single-molecule real-time, sequencing takes place on zero-mode waveguide chip. DNA polymerase at the bottom of the well, fluorescent nucleotides being added to the strand. Oxford nanopore technology relies on changes in ion flow as nucleotides pass through the nanopore.
FIGURE 2
FIGURE 2
The cost of sequencing a single human genome from 2001 to 2019. The y-axis represents the cost of sequencing a human genome, the x-axis is labelled from year 2001 to 2019. Moore’s Law predicts a biennial doubling of transistors on a microchip. The line drawn in the graph above illustrates Moore’s law prediction of the cost of sequencing per human genome. It is shown clearly that the cost has been consistently lower than predicted by Moore’s Law since 2008.
See this image and copyright information in PMC

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