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. 2020 Jun 20:28:51-62.
doi: 10.1016/j.jare.2020.06.014. eCollection 2021 Feb.

New biodegradable nanoparticles-in-nanofibers based membranes for guided periodontal tissue and bone regeneration with enhanced antibacterial activity

Dina Abdelaziz  1   2 , Amr Hefnawy  1 , Essam Al-Wakeel  2 , Abeer El-Fallal  2   3 , Ibrahim M El-Sherbiny  1
Affiliations

New biodegradable nanoparticles-in-nanofibers based membranes for guided periodontal tissue and bone regeneration with enhanced antibacterial activity

Dina Abdelaziz et al. J Adv Res. .

Abstract

Introduction: Guided tissue regeneration (GTR) and guided bone regeneration (GBR) are commonly used surgical procedures for the repair of damaged periodontal tissues. These procedures include the use of a membrane as barrier to prevent soft tissue ingrowth and to create space for slowly regenerating periodontium and bone. Recent approaches involve the use of membranes/scaffolds based on resorbable materials. These materials provide the advantage of dissolving by time without the need of surgical intervention to remove the scaffolds.

Objectives: This study aimed at preparing a new series of nanofibrous scaffolds for GTR/GBR applications with enhanced mechanical properties, cell adhesion, biocompatibility and antibacterial properties.

Methods: Electrospun nanofibrous scaffolds based on polylactic acid/cellulose acetate (PLA/CA) or poly(caprolactone) (PCL) polymers were prepared and characterized. Different concentrations of green-synthesized silver nanoparticles, AgNPs (1-2% w/v) and hydroxyapatite nanoparticles, HANPs (10-20% w/v) were incorporated into the scaffolds to enhance the antibacterial and bone regeneration activity.

Results: In-vitro studies showed that addition of HANPs improved the cell viability by around 50% for both types of nanofibrous scaffolds. The tensile properties were also improved through addition of 10% HANPs but deteriorated upon increasing the concentration to 20%. AgNPs significantly improved the antibacterial activity with 40 mm inhibition zone after 32 days. Additionally, the nanofibrous scaffolds showed a desirable degradation profile with losing around 40-70% of its mass in 8 weeks.

Conclusions: The obtained results show that the developed nanofibrous membranes are promising scaffolds for both GTR and GBR applications.

Keywords: GBR; GTR; Nanofibers; Nanoparticles; Periodontal.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

None
Graphical abstract
Scheme 1
Scheme 1
Development of a new series of electrospun nanoparticles-in-nanofibrous scaffolds for GTR/GBR applications with enhanced antibacterial and bone regeneration activity.
Fig. 1
Fig. 1
SEM micrographs of the developed PCL nanofibers after immersion in SBF for 2 and 8 weeks, respectively. (a, b) PCL, (c, d) PCL with 10% HANPs and 2% AgNPs and (e, f) PCL with 20% HANPs and 1% AgNPs.
Fig. 2
Fig. 2
SEM micrographs of PLA/CA-based nanofibers after immersion in SBF for 2 and 8 weeks, respectively. (a, b) PLA/CA, (c, d) PLA/CA with 10% HANPs and 2% AgNPs and (e, f) PLA/CA with 20% HANPs and 1% AgNPs.
Fig. 3
Fig. 3
Rate of biodegradation (measured as weight loss) and water absorption of the developed nanofibers after immersion in SBF for 8 weeks.
Fig. 4
Fig. 4
Release profiles of Ag+ from the electrospun nanofibers composites in PBS.
Fig. 5
Fig. 5
Mean and standard deviations of inhibition zones (mm) against (a) E. faecalis and (b) E. Coli.
See this image and copyright information in PMC

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