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. 2018 Aug 16;15(1):75-83.
doi: 10.21451/1984-3143-2017-AR939.

Effect of aflatoxin B1 on blood serum oestradiol-17β and progesterone concentrations during the luteal phase and the synchronized oestrus of goats

Georgios D Kourousekos  1 , Ekaterini K Theodosiadou  2 , Aristotelis G Lymberopoulos  3 , Sofia Belibasaki  4 , Constantin Boscos  1
Affiliations

Effect of aflatoxin B1 on blood serum oestradiol-17β and progesterone concentrations during the luteal phase and the synchronized oestrus of goats

Georgios D Kourousekos et al. Anim Reprod. .

Abstract

The effect of prolonged aflatoxin B1 (AFB1) administration on blood serum oestradiol-17β and progesterone concentrations in goats during the luteal phase and the synchronized oestrus was investigated. Thirty-six Greek indigenous primiparous goats were used, during the oestrus period; 12 goats received, per os, 50 μg (treated group T50) and 12 goats received 100 μg (treated group T100) AFB1/day/head, respectively, for approximately 1.5 month, while 12 goats served as controls (C). On day 36 of the experiment, each goat was injected, i.m, 0.5 ml prostaglandin F2α (PGF2α). Blood samples were collected from each goat twice a week, before PGF2α injection, as well as every 4 hours from the onset to the end of the synchronized oestrus. Oestradiol-17β and progesterone concentrations in blood serum were determined using radioimmunoassay. During the whole luteal(s) phase(s), linear regression analysis revealed a significant negative dependence (P < 0.05) of oestradiol-17β and a significant positive dependence (P < 0.05) of progesterone over group (C = 0, T50 = 50, T100 = 100), in a dose dependent manner. During the synchronized oestrus, multiple linear regression analysis revealed a significant negative dependence (P < 0.05) of oestradiol-17β, as well as a significant positive dependence (P < 0.05) of progesterone over group (C = 0, T50 = 50, T100 = 100) and over time (hours, from the onset to the end of the synchronized oestrus). No significant differences were noticed among the three groups, regarding the body weight of the goats from the onset to the end of AFB1 administration, the occurrence or the duration of the synchronized oestrus presented by the goats (P > 0.05). In conclusion, prolonged AFB1 administration at doses of 100 or even of 50 μg/day/head changes the hormonal pattern in blood during the luteal phase and the synchronized oestrus of goats, being in oestrus period.

Keywords: AFB1; hormones; mycotoxins; reproduction; ruminants.

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Figures

Figure 1
Figure 1. Oestradiol-17β concentration in blood serum (pg/mL) during the luteal(s) phase(s) of the goats [control group (C); treated group T50 (50 μg AFB1/goat/day); treated group T100 (100 μg AFB1/goat/day); (Mean ± SD)].
Figure 2
Figure 2. Progesterone concentration in blood serum (ng/mL) during the luteal(s) phase(s) of the goats [control group (C), treated group T50 (50 μg AFB1/goat/day); treated group T100 (100 μg AFB1/goat/day); (Mean ± SD)]. a,b,c Significant differences among the three groups at each time point studied (P < 0.05).
Figure 3
Figure 3. Oestradiol-17β concentration in blood serum (pg/mL) during the synchronized oestrus of the goats [control group (C); treated group T50 (50 μg AFB1/goat/day); treated group T100 (100 μg AFB1/goat/day); (Mean ± SD)]. a,b,c Significant differences among the three groups at each time point studied (P < 0.05).
Figure 4
Figure 4. Progesterone concentration in blood serum (ng/mL) during the synchronized oestrus of the goats [control group (C); treated group T50 (50 μg AFB1/goat/day); treated group T100 (100 μg AFB1/goat/day); (Mean ± SD)]. a,b,c Significant differences among the three groups at each time point studied (P < 0.05).
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