Characterization of the complete chloroplast genome sequence of Galinsoga parviflora and its phylogenetic implications

Abstract

Galinsoga parviflora is an invasive weed in southwest of Chinese agricultural systems and commonly used as medicine and food. In this study, the complete chloroplast genome of the G. parviflora was assembled from the whole genome Illumina sequencing data. The circular genome is 151,811 bp in size, which composed of one large single-copy (LSC) and one small single-copy (SSC) regions of 83,594 bp and 18,141 bp, respectively, and separated by a pair of inverted repeat (IR) regions of 25,038 bp each. It encodes a total of 113 gene species (80 protein-coding, 29 tRNA, and four rRNA species), in which 19 of them with double copies. The overall GC content is 37.7% while the GC content of the LSC, SSC, and IR regions are 35.8%, 31.3%, and 43.1%, separately. Phylogenetic analysis indicated that Galinsoga parviflora was closely related to Galinsoga quadriradiata.

Keywords: Galinsoga parviflora; chloroplast genome; phylogenetic analysis; weed.

Figure 1.

Phylogeny of 39 species within the family Asteraceae based on the maximum-likelihood (ML) of the concatenated chloroplast protein-coding sequences. The GTR + G + I model was employed as the best-fit nucleotide substitution model as suggested by MEGA7. The position of Galinsoga parviflora (GenBank accession number: MK737938) is shown in a box.