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. 2019 Jul 13;4(2):2459-2463.
doi: 10.1080/23802359.2019.1638321.

Molecular identification of the Danzhou chicken breed in China using DNA barcoding

Wenchuan Peng  1 , Hui Yang  1 , Keqi Cai  1 , Lu Zhou  1 , Zhen Tan  1 , Kebang Wu  1
Affiliations

Molecular identification of the Danzhou chicken breed in China using DNA barcoding

Wenchuan Peng et al. Mitochondrial DNA B Resour. .

Abstract

Mitochondrial cytochrome C oxidase subunit I (COI) has been used as a DNA barcode to identify population genetic diversity and distinguish animal species as it is variable enough to distinguish between species, yet suitably conserved. A new native chicken breed, named Danzhou chicken was discovered in Hainan, China in 2014, although identification is difficult by morphological examination alone. The mitochondrial COI genes of six chicken breeds, including four local and two imported breeds (Danzhou, Wenchang, Bawang, Beijing-You, Hy-Line Brown, and Ross) were compared and assessed in terms of their efficacy for DNA barcoding. The results showed that the number of COI gene variants in Danzhou chickens was less than those of other breeds, except Bawang and the genetic structure was relatively stable. The Kimura 2-parameter genetic distance between Danzhou chickens and the five other breeds was from ∼0.001 to 0.734. The genetic distance of the six breeds was ∼0.001-0.339, with that of Danzhou being the highest (0.339). Danzhou chickens clustered with Bawang and Wenchang chickens in the phylogenetic tree due to geographic closeness. Danzhou chickens could be identified more accurately using COI barcoding. Multiple molecular markers combined with morphological differences were more persuasive for identifying species.

Keywords: DNA barcode; Danzhou chickens; Molecular identification; cytochrome C oxidase subunit I (COI).

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Conflict of interest statement

All authors certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.

Figures

Figure 1.
Figure 1.
Phylogenetic tree of COI gene fragments of Danzhou and five other chicken breeds based on Kimura 2-parameter distance method.
See this image and copyright information in PMC

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References

    1. Bondoc OL, Gicana KRB, Hurtada JMUPA. 2015. Analysis of genetic diversity and distances of some dog (Canis lupus familiaris) breeds based on DNA barcodes. Philipp J Vet Animal Sci. 40:1–12.
    1. Donald KM, Kennedy M, Spencer HG. 2005. The phylogeny and taxonomy of austral monodontine topshells (Mollusca: Gastropoda: Trochidae), inferred from DNA sequences. Mol Phylogenet Evol. 37:474–483. - PubMed
    1. Gao Y, Tu Y, Tong H, Wang K, Chen K, Gu R. 2007. DNA barcoding application of mtDNA CO I gene in identifying six indigenous chicken breeds in China. J Agric Biotechnol.
    1. Hajibabaei M, Janzen DH, Burns JM, Hallwachs W, Hebert PD. 2006. DNA barcodes distinguish species of tropical Lepidoptera. Proc Natl Acad Sci USA. 103:968–971. - PMC - PubMed
    1. Hebert PD, Penton EH, Burns JM, Janzen DH, Hallwachs W. 2004. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proc Natl Acad Sci USA. 101:14812–14817. - PMC - PubMed