Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Oct 21;4(2):3675-3676.
doi: 10.1080/23802359.2019.1678437.

The complete chloroplast genome sequence of Cynanchum forrestii Schltr. (Asclepiadaceae) and its phylogenetic analysis

Jie Zhang  1 , Dequan Zhang  1   2   3
Affiliations

The complete chloroplast genome sequence of Cynanchum forrestii Schltr. (Asclepiadaceae) and its phylogenetic analysis

Jie Zhang et al. Mitochondrial DNA B Resour. .

Abstract

Cynanchum forrestii is a folk medicinal plant in southwest China. In this study, we sequenced complete chloroplast (cp) genome sequence of C. forrestii to investigate its phylogenetic relationship. The whole cp genome of C. forrestii was 159,917 bp in length with 43.5% overall GC content, including a large single-copy (LSC) region of 91,189 bp and a small single-copy (SSC) region of 19,972 bp, which were separated by a pair of inverted repeats (IRs) of 24,378 bp. The cp genome contained 112 genes, including 78 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. The phylogenetic analysis based on cp genome sequences showed that Cynanchum was closely related with Asclepias and Calotropis.

Keywords: Cynanchum forrestii; complete chloroplast genome; medicinal plant; phylogenetic analysis.

PubMed Disclaimer

Conflict of interest statement

No potential conflict of interest was reported by the authors.

Figures

Figure 1.
Figure 1.
Phylogenetic position of C. forrestii inferred by the neighbour-joining (NJ) analysis based on 19 sequences. Numbers in the nodes are the bootstrap values from 1000 replicates.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Bolger AM, Lohse M, Usadel B. 2014. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 30:2114–2120. - PMC - PubMed
    1. Chen JJ, Zhang ZX, Zhou J. 1989. The chemical composition of Cynanchum forrestii Schltr. Acta Bot Yunnan. 11:471–475.
    1. Doyle J. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull. 19:11–15.
    1. Jin JJ, Yu WB, Yang JB, Song Y, Yi TS, Li DZ. 2018. GetOrganelle: a simple and fast pipeline for de novo assembly of a complete circular chloroplast genome using genome skimming data. bioRxiv. 1–11.
    1. Katoh K, Standley DM. 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 30:772–780. - PMC - PubMed

LinkOut - more resources