The complete mitochondrial genome of Poecilia formosa (Poecilia, Cyprinodontidae) and phylogenetic studies of cyprinodontiformes

Abstract

We report the complete mitochondrial genome sequence of Poecilia formosa. The genome is found to be 16636 bp in length and has a base composition of A (29.59%), G (14.61%), C (28.26%), and T (27.54%). Similar to other Poecilia species, it contains a typically conserved structure including 13 protein-coding genes, 2 rRNA genes, 1 control region (D-loop), and 22tRNA genes. The proportion of coding sequences with a total length of 11,533 bp is 69.33%, which encodes 3837 amino acids. All protein-coding genes started with Met, ND1, CO1, ATP8, ATP6, CO3, ND4L, ND5, ND6 and CytB ended by TAA as a stop codon, ND2 and ND3 ended by TAG as a stop codon, CO3 and ND4 ended by a single T. The lengths of 12S ribosomal RNA is 948 bp, ranging from 70 bp to 1018 bp, and the lengths of 16S ribosomal RNA is 1674 bp, ranging from 1090 bp to 2764 bp. The length of control region is 879 bp, ranging from 15757 bp to 16636 bp, respectively. The complete mitochondrial genome sequence provided here would be useful for further understanding the evolution of ratite and conservation genetics of Poecilia formosa.

Keywords: Poecilia formosa; evolutionary relationships; mitochondrial genome.

Figure 1.

Phylogenetic tree derived from NJ based on concatenated nucleotide sequences of twelve PCGs. Including Poecilia reticulata, Poecilia sphenops, Poecilia formosa, Poeciliopsis monacha, Xiphophorus hellerii, Orestias ascotanensis, Cyprinodon diabolis, Cyprinodon veriegatus isolate, Cyprinodon nevadensis amargosae, Austrolebias charrua voucher, Kryptolebias hemaphroditus, Rivulus marmoratus,.Kryptolebias marmoratus.