Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jan 10;5(1):478-479.
doi: 10.1080/23802359.2019.1704656.

Chloroplast characterization and phylogenetic relationship of Cymbidium aloifolium (Orchidaceae)

Affiliations

Chloroplast characterization and phylogenetic relationship of Cymbidium aloifolium (Orchidaceae)

Juan Chen et al. Mitochondrial DNA B Resour. .

Abstract

Cymbidium aloifolium is an epiphytic orchid with high medicinal and ornamental value. In order to get a deeper understanding of C. aloifolium, we determined the complete chloroplast genome of C. aloifolium by Illumina sequencing data. The length of this genome is 157,328 bp, including a couple of inverted repeat (IR) regions of 26,829 bp, a large single-copy (LSC) region of 85,793 bp, and a small single-copy (SSC) region of 17,877 bp. The chloroplast genome comprised of 139 genes, including 78 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. In addition, the phylogenetic analysis based on 17 chloroplast genomes of Orchidaceae indicated that C. mannii was closely related to C. aloifolium. This study will provide more valuable information for the classification and phylogenetic research of Cymbidium genus.

Keywords: Cymbidium aloifolium; Illumina sequencing; Orchid; chloroplast genome.

PubMed Disclaimer

Conflict of interest statement

No potential conflict of interest was reported by the authors.

Figures

Figure 1.
Figure 1.
The maximum likelihood tree based on 17 complete chloroplast genome sequences of Orchidaceae, and the position of C. aloifolium is shown in bold.

References

    1. Ankenbrand MJ, Pfaff S, Terhoeven N, Qureischi M, Gündel M, Weiß CL, Hackl T, Förster F. 2018. chloroExtractor: extraction and assembly of the chloroplast genome from whole genome shotgun data. JOSS. 3(21):464–466.
    1. Chen S, Zhou Y, Chen Y, Gu J. 2018. fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics. 34(17):i884–i890. - PMC - PubMed
    1. Deb CR, Pongener A. 2011. Asymbiotic seed germination and in vitro seedling development of Cymbidium aloifolium (L.) Sw.: a multipurpose orchid. J Plant Biochem Biot. 20(1):90–95.
    1. Hossain MM, Sharma M, Pathak P. 2009. Cost effective protocol for in vitro mass propagation of Cymbidium aloifolium (L.) Sw. – A medicinally important orchid. Engineering in Life Sciences. 9(6):444–453.
    1. Howlader MA, Alam M, Ahmed KT, Khatun F, Apu AS. 2011. Antinociceptive and anti-inflammatory activity of the ethanolic extract of Cymbidium aloifolium (L.). Pakistan Journal of Biological Sciences: PJBS. 14(19):909–911. - PubMed

LinkOut - more resources