The plastid genome of Herpetospermum pedunculosum (Cucurbitaceae), an endangered traditional Tibetan medicinal herbs
- PMID: 33366618
- PMCID: PMC7748728
- DOI: 10.1080/23802359.2019.1703603
The plastid genome of Herpetospermum pedunculosum (Cucurbitaceae), an endangered traditional Tibetan medicinal herbs
Abstract
Herpetospermum pedunculosum (Ser.) C. B. Clarke is an important traditional Tibetan medicinal plants in the genus of Herpetospermum, Cucurbitaceae. To better determine its phylogenetic location with respect to the other Cucurbitaceae species, the complete plastome of H. pedunculosum will be reported, which is the first species with plastid genome sequence in the genus of Herpetospermum. Its whole genome is 156,531 bp in length, consisting of a pair of inverted repeats (IRs) of 26,147 bp, one large single-copy (LSC) region of 85,878 bp, and one small single-copy (SSC) region of 18,359 bp. There are 128 genes, including 83 protein-coding genes, 36 transfer RNA (tRNA) genes, and 8 ribosomal RNA (rRNA) genes in the plastome. Phylogenetic analysis based on 13 complete plastomes of Cucurbitaceae species showed sisterhood of H. pedunculosum and a clade containing Trichosanthes kirilowii and Hodgsonia macrocarpa, suggesting the close relationship between tribe Schizopeponeae and tribe Sicyoeae in the family Cucurbitaceae.
Keywords: Herpetospermum pedunculosum; chloroplast genome; phylogenetic analysis Cucurbitaceae.
© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Conflict of interest statement
No potential conflict of interest was reported by the authors.
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References
-
- Doyle JJ, Doyle JL. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bulletin. 19:11–15.
-
- Kim HC, Peng M, Liu S, Wang YJ, Li ZS, Zhao SD, Li SH, Quan H, Luo QX, Meng FJ. 2018. Comparative proteomic analysis reveals the adaptation of Herpetospermum pedunculosum to an altitudinal gradient in the Tibetan Plateau. Biochem Syst Ecol. 80:1–10.
-
- Kocyan A, Zhang LB, Schaefer H, Renner SS. 2007. A multi-locus chloroplast phylogeny for the Cucurbitaceae and its implications for character evolution and classification. Mol Phylogenet Evol. 44(2):553–577. - PubMed
-
- Ma L, Yang LM, Zhao JJ, Wei JJ, Kong XX, Wang CT, Zhang XM, Yang YP, Hu XY. 2015. Comparative proteomic analysis reveals the role of hydrogen sulfide in the adaptation of the alpine plant Lamiophlomis rotata to altitude gradient in the Northern Tibetan Plateau. Planta. 241(4):887–906. - PubMed
-
- Ma Y, Ma Y, Wang H, Wang R, Meng F, Dong Z, Wang G, Lan X, Quan H, Liao Z, et al. . 2019. Cytotoxic lignans from the stems of Herpetospermum pedunculosum. Phytochemistry. 164:102–110. - PubMed
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