The complete mitochondrial genome of Riccia fluitans L. (Ricciaceae, Marchantiophyta): investigation of intraspecific variations on mitochondrial genomes of R. fluitans

Abstract

Riccia fluitans L. is the most common species in Riccia genus. To investigate intraspecific variations on mitochondrial genomes of R. fluitans, we completed mitochondrial genome of R. fluitans. Its length is 185,640 bp, longer than that of NC_043906 by 19 bp and it contains 74 genes (42 protein-coding genes, 3 rRNAs, 28 tRNAs, and 1 pseudogene). 18 single nucleotide polymorphisms (SNPs) and 19 insertions and deletions are identified, higher than that of Marchantia polymorpha subsp. ruderalis. One non-synonymous SNP is found in ccmFN. Phylogenetic trees show that R. fluitans is clustered with Dumortiera hirsuta, requiring additional mitogenome to clarify the phylogenetic relationship.

Keywords: Riccia fluitans; Ricciaceae; ccmFN; intraspecific variation; mitochondrial genome.

Figure 1

Neighbor joining (bootstrap repeat is 10,000) and maximum likelihood (bootstrap repeat is 1,000) phylogenetic trees of 22 complete mitochondrial genomes: Riccia fluitans (MN927134 in this study and NC_043906), Marchantia polymorpha subsp. ruderalis (NC_037508 and MK202951), Marchantia paleacea (NC_001660), Pleurozia purpurea (NC_013444), Aneura pinguis (NC_026901), Dumortiera hirsuta (NC_042873), Calypogeia neogaea (NC_035980), Calypogeia integristipula (NC_035977), Calypogeia suecica (MF401631), Calypogeia arguta (MF401630), Calypogeia integristipula (MF401629), Riccardia planiflora (MK318535), Solenostoma sphaerocarpum (NC_043909), Porella platyphylla (NC_043908), Ptilidium ciliare (NC_043907), Metzgeria furcata (NC_043888), Haplomitrium hookeri (NC_043889), Tritomaria quinquedentata (NC_037041), Gymnomitrion concinnatum (NC_040132), Calypogeia integristipula (NC_035977), Treubia lacunose (NC_016122), and Sphagnum palustre (NC_024521) as an outgroup. The numbers above branches indicate bootstrap support values of maximum likelihood and neighbor-joining phylogenetic trees, respectively.