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. 2020 Dec;49(4):567-573.
doi: 10.1111/vcp.12926. Epub 2020 Dec 27.

Establishment of normal reference intervals in dogs using a viscoelastic point-of-care coagulation monitor and its comparison with thromboelastography

Yekaterina Buriko  1 , Kenneth Drobatz  1 , Deborah C Silverstein  1
Affiliations

Establishment of normal reference intervals in dogs using a viscoelastic point-of-care coagulation monitor and its comparison with thromboelastography

Yekaterina Buriko et al. Vet Clin Pathol. 2020 Dec.

Abstract

Background: Viscoelastic coagulation devices are a useful adjunct to the evaluation of hemostasis in veterinary patients. VCM Vet is a point-of-care device that is simple in operation and could be used to diagnose and trend hemostatic abnormalities in sick patients. VCM Vet does not use activators.

Objectives: We aimed to establish reference intervals (RIs) for VCM Vet in a healthy adult canine population and concurrently perform thromboelastographic (TEG) analysis on these samples with and without tissue factor (TF) activation for RI comparisons.

Methods: Duplicate VCM Vet tests were performed immediately upon sample collection. Two concurrent TEG tests were performed on the remaining blood, one citrated, untreated (CU), and one activated with TF at a 1:3600 dilution.

Results: Fifty-two dogs were enrolled in the study. The following RIs were generated for VCM Vet machine 1 and 2, respectively: clot time (CT) (seconds) 163-480 and 172-457; clot formation time (CFT) (seconds) 104-288 and 94-252, α-angle (degrees) 41-65 and 44-66, and maximum clot firmness (MCF) (no units) 27-43 and 30-46. Moderate to good correlations were observed between the two machines with Lin's concordance correlation coefficients of 0.51-0.9 and a P < 0.002. TEG RIs were similar to previously reported values.

Conclusions: VCM Vet RIs were generated. Each VCM Vet device should have a unique RI established due to inter-device variability. Direct correlations of VCM Vet values with TEG parameters were not performed due to the narrow range of the normal values and the need to evaluate patients with a wide range of hemostatic abnormalities.

Keywords: analyzer; clotting; device; hemostasis.

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References

REFERENCES

    1. Hans GA, Besser MW. The place of viscoelastic testing in clinical practice. Br J Haematol. 2016;173(1):3748.
    1. Goggs R, Brainard B, de Laforcade AM, et al. Partnership on Rotational ViscoElastic Test Standardization (PROVETS): evidence-based guidelines on rotational viscoelastic assays in veterinary medicine. J Vet Emerg Crit Care. 2014;24(1):1-22.
    1. McMichael MA, Smith SA. Viscoelastic coagulation testing: technology, applications, and limitations. Vet Clin Pathol. 2011;40(2):140-153.
    1. Wikkelso A, Wetterslev J, Moller AM, Afshari A. Thromboelastography (TEG) or rotational thromboelastometry (ROTEM) to monitor haemostatic treatment in bleeding patients: a systematic review with meta-analysis and trial sequential analysis. Anaesthesia. 2017;72(4):519-531.
    1. Levrat A, Gros A, Rugeri L, et al. Evaluation of rotation thrombelastography for the diagnosis of hyperfibrinolysis in trauma patients. Br J Anaesth. 2008;100(6):792-797.

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