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. 2020 Nov 9;1(3):100169.
doi: 10.1016/j.xpro.2020.100169. eCollection 2020 Dec 18.

Sensitization of Non-permissive Laboratory Mice to SARS-CoV-2 with a Replication-Deficient Adenovirus Expressing Human ACE2

Lok-Yin Roy Wong  1 , Kun Li  2 , Jing Sun  3 , Zhen Zhuang  3 , Jincun Zhao  3   4 , Paul B McCray Jr  1   2 , Stanley Perlman  1   2
Affiliations

Sensitization of Non-permissive Laboratory Mice to SARS-CoV-2 with a Replication-Deficient Adenovirus Expressing Human ACE2

Lok-Yin Roy Wong et al. STAR Protoc. .

Abstract

Common laboratory mice such as BALB/c and C57BL/6 mice are not permissive to SARS-CoV2 infection. Sensitization of laboratory mice with Adenovirus expressing human ACE2 (Ad5-hACE2) provides a rapid model for testing viral intervention in vivo. Despite the lack of lethal outcome, Ad5-hACE2-sensitized mice show 20% weight loss on average upon viral challenge with infectious virus being detected at the site of sensitization. This protocol describes the sensitization and subsequent infection of common laboratory mice for use in testing anti-viral interventions. For complete details on the use and execution of this protocol, please refer to Sun et al. (2020).

Keywords: Immunology; Model Organisms.

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Conflict of interest statement

The authors declare no competing interests.

Figures

None
Graphical abstract
Figure 1
Figure 1
Schematic Diagram Showing the Organization of the Ad5-hACE2 Vector for Generation of Ad5-hACE2
See this image and copyright information in PMC

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