Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Aug;26(8):708-712.

[Correlation of sperm DNA fragmentation index with age and semen parameters and its influence on the outcomes of IVF-ET]

[Article in Chinese]
Qiang-Xiang Luo  1   2 , Yong-Bin Liao  1   2 , Chang-Ping Huang  1   2 , Jiang-Hua Guo  1 , Xiao-Dong Liang  1
Affiliations
  • PMID: 33377731

[Correlation of sperm DNA fragmentation index with age and semen parameters and its influence on the outcomes of IVF-ET]

[Article in Chinese]
Qiang-Xiang Luo et al. Zhonghua Nan Ke Xue. 2020 Aug.

Abstract

Objective: To explore the correlation of the sperm DNA fragmentation index (DFI) with age and other semen parameters in infertile men and its influence on the outcomes of in vitro fertilization and embryo transplantation (IVF-ET).

Methods: Semen samples were obtained from 6 162 infertile males in our hospital between July 2017 and December 2018. Sperm concentration, the percentages of progressively motile sperm (PMS) and morphologically normal sperm (MNS) and sperm DFI were determined by computer-assisted semen analysis, modified Papanicolaou staining and sperm chromatin structure assay, respectively. According to the sperm DFI, the samples were divided into three groups: DFI≤15%, 15%<DFI<30%, and DFI≥30%, followed by analysis of the correlation of sperm DFI with age and other semen parameters. The clinical data were also collected from 1 206 couples undergoing IVF-ET between January 2015 and December 2018, who fell into four DFI groups: DFI≤10%, 10%<DFI≤20%, 20%<DFI≤30%), and DFI>30%. Comparisons were made among different groups in the rates of fertilization, embryo cleavage, embryo formation and pregnancy.

Results: Spearman correlation analysis showed that DFI was correlated positively with age (r = 0.508, P < 0.05), but negatively with sperm concentration (r = －0.155, P < 0.05) and the percentages of PMS (r = －0.111, P < 0.05) and MNS (r = －0.315, P < 0.05). With sperm DFI >20%, the clinical pregnancy rate of IVF-ET was significantly reduced, while with sperm DFI >30%, the rate of available embryos markedly decreased and that of biochemical pregnancy remarkably increased. No correlation was found between sperm DFI and the rates of fertilization, embryo cleavage and high-quality embryos in IVF-ET.

Conclusions: In infertile males, sperm DFI is correlated positively with age but negatively with sperm concentration and the percentages of PMS and MNS, and it significantly affects the outcomes of IVF-ET.

Keywords: age; in vitro fertilization and embryo transplantation; semen parameters; sperm DNA fragmentation index.

PubMed Disclaimer

LinkOut - more resources