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. 2020 Sep 1;90(5):702-706.
doi: 10.2319/090219-574.1.

MicroRNA-34 expression in gingival crevicular fluid correlated with orthodontic tooth movement

Bin Zhang, Li Yang, Weilong Zheng, Ting Lin

MicroRNA-34 expression in gingival crevicular fluid correlated with orthodontic tooth movement

Bin Zhang et al. Angle Orthod. .

Abstract

Objectives: To explore the expression of miR-34a and its effect on expression of matrix metalloproteinases (MMPs) during orthodontic tooth movement (OTM).

Materials and methods: Twenty patients, age 12-18 years old, who underwent orthodontic treatment were enrolled. The expression of miR-34a and MMPs (MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, and MMP-14) were detected in gingival crevicular fluid by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction at different time points. The miR-34a mimics or inhibitors were transfected into human periodontal ligament (hPDL) cells, and the MMP expression was measured by ELISA.

Results: The miR-34 expression in GCF on both the tension and pressure sides after orthodontic treatment were significantly downregulated, while the levels of MMPs were significantly upregulated compared with baseline level. The levels of miR-34 and MMPs returned to baseline level 3 months after orthodontic treatment. The expression of miR-34 was negatively correlated with the expression of MMP-2, MMP-9, and MMP-14. After transfection with miR-34, the MMP-2, MMP-9, and MMP-14 expression by hPDL cells were significantly downregulated compared with miR-control and miR-34 inhibitor.

Conclusions: Downregulated miR-34 expression was positively correlated with MMP-2, MMP-9, and MMP-14 expression. The miR-34a transfection into hPDL cells inhibited expression of MMPs. The results suggest that miR-34a is involved in expression of MMPs during OTM.

Keywords: Human periodontal ligament; Matrix metalloproteinases; MiR-34; Orthodontic tooth movement.

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Figures

Figure 1.
Figure 1.
The expression of MMP-2, MMP-9, and MMP-14 by periodontal ligament (PDL) cells after being transfected with miR-34a mimics or inhibitor.
See this image and copyright information in PMC

References

    1. Huang H, Williams RC, Kyrkanides S. Accelerated orthodontic tooth movement: molecular mechanisms. Am J Orthod Dentofacial Orthop. 2014;146:620–632. - PubMed
    1. Alhashimi N, Frithiof L, Brudvik P, Bakhiet M. Orthodontic tooth movement and de novo synthesis of proinflammatory cytokines. Am J Orthod Dentofac Orthop. 2001;119:307–312. - PubMed
    1. Kapoor P, Kharbanda OP, Monga N, Miglani R, Kapila S. Effect of orthodontic forces on cytokine and receptor levels in gingival crevicular fluid: a systematic review. Prog Orthod. 2014;15:65. - PMC - PubMed
    1. Ketting RF. MicroRNA biogenesis and function: an overview. Adv Exp Med Biol. 2011;700:1–4. - PubMed
    1. PI C, Li YP, Zhou X, Gao B. The expression and function of microRNAs in bone homeostasis. Front Biosci. 2015;20:119–138. - PubMed