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. 2021 Jan 1;6(1):1.
doi: 10.1038/s41392-020-00451-w.

A mouse model for SARS-CoV-2-induced acute respiratory distress syndrome

Weiqi Hong  1 , Jingyun Yang  1 , Zhenfei Bi  1 , Cai He  1 , Hong Lei  1 , Wenhai Yu  2 , Yun Yang  2 , Changfa Fan  3 , Shuaiyao Lu  4 , Xiaozhong Peng  5   6 , Xiawei Wei  7
Affiliations

A mouse model for SARS-CoV-2-induced acute respiratory distress syndrome

Weiqi Hong et al. Signal Transduct Target Ther. .
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
The characterization and treatment of a mouse model for SARS-CoV-2-induced acute respiratory distress syndrome (ARDS). a The establishment of a mouse model of ARDS. Illustration for routine tracheotomy and intratracheal instillation of SARS-CoV-2 in mouse (4 × 105 PFU). b Morphology of the lung from the mouse 5 days after intratracheal instillation of SARS-CoV-2 (4 × 105 PFU). c, d The pulmonary pathological changes in mice after intratracheal instillation of SARS-CoV-2 characterized by H&E staining. e TUNEL labeling was used to detect the cell apoptosis in lung tissue as described in Supplementary Materials. f The neutrophils were predominant cell infiltration with 60% of lung tissues consolidated as stained by H&E staining. g The neutrophils were stained positive for Ly6G. h Apparent hemorrhage, with the red blood cells filling the alveolar spaces in the section. i Inflammatory cells, red blood cells, and proteinaceous debris were found in the bronchioles. j Proteinaceous and cellular debris were found in the alveolar space (arrow). k Hyaline membranes-like changes lining alveolar cavities (arrow). l The thrombi were present in the vessels (arrow). m The immunostaining of SARS-CoV-2 spike protein in the lung section of a mouse with SARS-CoV-2-induced ARDS, as described in Supplementary Materials. n Pathological scores for mice from SARS-CoV-2-induced ARDS group or dexamethasone-treated group according to the scoring system (n = 3 mice). o The lung weights of mice from SARS-CoV-2-induced ARDS group or dexamethasone-treated group (n = 3). Mice were treated with dexamethasone as described in Supplementary Materials. p Cytokines changes in mice from SARS-CoV-2-induced ARDS group or dexamethasone-treated group (n = 3). Cytokines were measured as described in Supplementary Materials. Scale bars represent 2000 μm (c) or 20 μm (d–m). Statistical analysis of pathological score (n) and lung weight (o) was analyzed by Student’s unpaired t test, and cytokines changes (p) data were analyzed by one-way ANOVA. All error bars represent SEM about the mean. **P < 0.01
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