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. 2021 Jan;39(1):13-17.
doi: 10.1038/s41587-020-00762-x.

The Organoid Cell Atlas

Christoph Bock  1   2 , Michael Boutros  3 , J Gray Camp  4 , Laura Clarke  5 , Hans Clevers  6   7 , Juergen A Knoblich  8 , Prisca Liberali  9 , Aviv Regev  10   11 , Anne C Rios  6   12   13 , Oliver Stegle  14   15 , Hendrik G Stunnenberg  12 , Sarah A Teichmann  16   17 , Barbara Treutlein  18 , Robert G J Vries  19 ; Human Cell Atlas ‘Biological Network’ Organoids
Affiliations

The Organoid Cell Atlas

Christoph Bock et al. Nat Biotechnol. 2021 Jan.
No abstract available

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Conflict of interest statement

C.B. is cofounder and scientific advisory board member of Aelian Biotechnology and an inventor on patent applications relating to genome technology. H.C. is an inventor on patents held by the Royal Netherlands Academy of Arts and Sciences that cover organoid technology (full disclosure: https://www.uu.nl/staff/JCClevers/Additional%20functions). J.A.K. is an inventor on several patents involving pluripotent stem cell–based organoid technology, and cofounder and scientific advisory board member of a:head bio. A.R. is cofounder of Celsius Therapeutics; equity holder of Imunitas; scientific advisory board member of Syros Pharmaceuticals, Thermo Fisher Scientific, Asimov and NeoGene Therapeutics; and co-inventor on patent applications filed by the Broad Institute relating to single-cell genomics. S.A.T. consults for Genentech and Roche, and serves on the scientific advisory board for Biogen, Foresite Labs and GlaxoSmithKline. R.G.J.V. is an inventor on a patent involving adult stem cell–based organoid technology and a full-time employee of the Foundation Hubrecht Organoid Technology (HUB), which holds an exclusive license on organoid technology patents.

Figures

Fig. 1
Fig. 1. Combining organoids and single-cell technology for biomedical discovery and regenerative therapy.
a, Organoids can be derived from adult stem cells, which are already committed to a specific cell lineage, tissue and/or organ, or from pluripotent cells, which can give rise to a broad set of cell types but requires more complex differentiation protocols. b, Human organoids are broadly useful for in vitro modeling of biological functions, drug testing and regenerative medicine. c, Key challenges for advancing organoid research include characterization and validation of organoids, development of new organoid protocols, and applications of organoids in basic biology and biomedical research. d, Single-cell technologies promise to advance organoid research by enabling systematic validation of the derived organoids, by informing organoid protocol development, and by providing a high-resolution readout for functional perturbation experiments in human organoids.
Fig. 2
Fig. 2. Connecting single cells in human organoids and in primary tissue samples via the Organoid Cell Atlas.
a, Single-cell profiling of human organoids (left) and of human primary tissue samples (right) provides complementary information. Data integration between single-cell profiles from organoids and primary tissues makes it possible to investigate the same cell type in both contexts, allowing each approach to play to its strengths. b, The Organoid Cell Atlas Portal will implement key features for analyzing and interpreting single-cell data from human organoids in the biological context provided by HCA profiles of their in vivo counterparts.
See this image and copyright information in PMC

References

    1. Clevers H. Cell. 2016;165:1586–1597. doi: 10.1016/j.cell.2016.05.082. - DOI - PubMed
    1. Lancaster MA, Knoblich JA. Science. 2014;345:1247125. doi: 10.1126/science.1247125. - DOI - PubMed
    1. Rossi G, Manfrin A, Lutolf MP. Nat. Rev. Genet. 2018;19:671–687. doi: 10.1038/s41576-018-0051-9. - DOI - PubMed
    1. Regev A, et al. eLife. 2017;6:e27041. doi: 10.7554/eLife.27041. - DOI - PMC - PubMed
    1. Rozenblatt-Rosen O, Stubbington MJT, Regev A, Teichmann SA. Nature. 2017;550:451–453. doi: 10.1038/550451a. - DOI - PubMed

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