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. 1977:(259):17-24.

Quantitative determination of free amino acids by gas-liquid chromatography with special reference to yeasts

I Tunblad-Johansson
  • PMID: 333865

Quantitative determination of free amino acids by gas-liquid chromatography with special reference to yeasts

I Tunblad-Johansson. Acta Pathol Microbiol Scand Suppl. 1977.

Abstract

A gas-liquid chromatographic method for quantitative determination of free amino acids in yeasts is described. The amino acids are extracted with boiling water and separated from interfering substances on a cation-exchange resin. They are analyzed as their n-propyl N-acetyl esters. The best separation of the derivatives was achieved when using glass columns containing a mixture of three polar phases.

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