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. 2022:273:223-244.
doi: 10.1007/164_2020_425.

In Vivo Studies of Drug BBB Transport: Translational Challenges and the Role of Brain Imaging

Stina Syvänen  1 , Margareta Hammarlund-Udenaes  2 , Irena Loryan  2
Affiliations

In Vivo Studies of Drug BBB Transport: Translational Challenges and the Role of Brain Imaging

Stina Syvänen et al. Handb Exp Pharmacol. 2022.

Abstract

Drug delivery to the brain is challenging to study due to the complexity of the barriers of the central nervous system (CNS). The present chapter describes and compares experimental methods such as microdialysis, two-photon laser scanning fluorescence microscopy and positron emission tomography (PET) that can be used for in vivo studies of drug transport across the blood-brain barrier (BBB). The selection of appropriate method is based on the research question, and the different methods will in most cases provide complementary information. Attention is also given to the fact that the BBB might undergo changes in integrity, protein expression and other morphological alterations as a result of disease. The use of animal models of human disease is therefore also discussed. Special emphasis is given to translational aspects of the different methods and readouts.

Keywords: Brain barriers; In vivo studies; Microdialysis; Molecular imaging; Positron emission tomography (PET); Translation.

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References

    1. Abanades S, van der Aart J, Barletta JA, Marzano C, Searle GE, Salinas CA, Ahmad JJ, Reiley RR, Pampols-Maso S, Zamuner S, Cunningham VJ, Rabiner EA, Laruelle MA, Gunn RN (2011) Prediction of repeat-dose occupancy from single-dose data: characterisation of the relationship between plasma pharmacokinetics and brain target occupancy. J Cereb Blood Flow Metab 31(3):944–952 - PubMed - DOI
    1. Abbott NJ (2004) Evidence for bulk flow of brain interstitial fluid: significance for physiology and pathology. Neurochem Int 45(4):545–552 - PubMed - DOI
    1. Abbott NJ, Pizzo ME, Preston JE, Janigro D, Thorne RG (2018) The role of brain barriers in fluid movement in the CNS: is there a ‘glymphatic’ system? Acta Neuropathol 135(3):387–407 - PubMed - DOI
    1. Ahn JH, Cho H, Kim J-H, Kim SH, Ham J-S, Park I, Suh SH, Hong SP, Song J-H, Hong Y-K, Jeong Y, Park S-H, Koh GY (2019) Meningeal lymphatic vessels at the skull base drain cerebrospinal fluid. Nature 572(7767):62–66 - PubMed - DOI
    1. Andersson PB, Perry VH, Gordon S (1992) The acute inflammatory response to lipopolysaccharide in CNS parenchyma differs from that in other body tissues. Neuroscience 48(1):169–186 - PubMed - DOI

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